Yao Liang-Xue, Han Jia-Xin, Wei Pei-Yun, Hou Si-Bo, He Xiao-Jian, Zhang Rui-Ling, DU Ai-Lin
Department of Physiology and Neurobiology, Sino-UK Joint Laboratory for Brain Function and Injury, Henan Provincial Key Laboratory of Brain Research, Xinxiang 453003.
The Second Affiliated Hospital of Xinxiang Medical University, Henan Key Laboratory of Biopsychology, Xinxiang Medical University, Xinxiang 453003, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Mar;36(2):138-142. doi: 10.12047/j.cjap.5902.2020.031.
To investigate the effects of butylphthalide (NBP) on learning and memory related ability, hydrogen sulfide (HS) content in hippocampus and amygdala, cystathionine-β-synthase (CBS) expression and mitochondrial ATPase activity in rats with chronic alcoholism. Ninety SD male rats were randomly divided into three groups: normal control group (NC), model group (M) and butylphthalide remedy group (BR). Except for the control group, the water solution containing 6% (v/v) alcohol was used as the sole source of drinking water in the other two groups. After 14 days of feeding, the butylphthalide remedy group was injected with NBP intraperitoneally at the dose of 5 mg/kg once a day for 14 consecutive days, and the remaining two groups were injected with the same dose of normal saline. The control group subsequently used the Morris water maze method to observe and record the animals after entering the water. The time required for the underwater platform, their strategies and their swimming trajectories could analyze and infer the animal's ability to learn and remember. HS concentration, CBS expression and mitochondrial ATPase activity in hippocampus and amygdale were dectected. Compared with NC group, the latency period and swimming distance of M group were increased, the content of HS and the mean optical density of CBS in hippocampus and amygdala were increased, and the activity of mitochondrial ATPase in hippocampus and amygdala was decreased significantly (＜0. 01) . Compared with the M group, the latency period and swimming distance of learning and memory performance of BR group were decreased, the content of HS and the mean optical density of CBS in hippocampus and amygdala were decreased, and the activity of mitochondrial ATPase in hippocampus and amygdala was increased significantly (＜0. 01) . NBP can alleviate the effect of ethanol on learning and memory in rats, which may be related to the effect of NBP on the concentration of HS and the expression of CBS in the amygdala of hippocampus and the increase of ATPase activity.
为探讨丁苯酞（NBP）对慢性酒精中毒大鼠学习记忆相关能力、海马和杏仁核中硫化氢（HS）含量、胱硫醚-β-合酶（CBS）表达及线粒体ATP酶活性的影响。将90只SD雄性大鼠随机分为三组：正常对照组（NC）、模型组（M）和丁苯酞治疗组（BR）。除对照组外，其他两组用含6%（v/v）酒精的水溶液作为唯一饮用水源。喂养14天后，丁苯酞治疗组每天腹腔注射5mg/kg的NBP，连续14天，其余两组注射相同剂量的生理盐水。对照组随后采用Morris水迷宫法观察并记录动物入水后的情况。通过动物找到水下平台所需时间、策略及游泳轨迹来分析和推断其学习记忆能力。检测海马和杏仁核中HS浓度、CBS表达及线粒体ATP酶活性。与NC组相比，M组潜伏期和游泳距离增加，海马和杏仁核中HS含量及CBS平均光密度增加，海马和杏仁核中线粒体ATP酶活性显著降低（＜0.01）。与M组相比，BR组学习记忆成绩的潜伏期和游泳距离降低，海马和杏仁核中HS含量及CBS平均光密度降低，海马和杏仁核中线粒体ATP酶活性显著增加（＜0.01）。NBP可减轻乙醇对大鼠学习记忆的影响，这可能与NBP对海马杏仁核中HS浓度、CBS表达的影响及ATP酶活性增加有关。
