Zhu T R, Cao J, Hong J W, Li J
Xuzhou Medical University, Xuzhou 221004, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020 Jul 20;38(7):481-484. doi: 10.3760/cma.j.cn121094-20190917-00380.
To investigate the effect of perfluorooctane sulfonate (PFOS) on inflammatory factors in human placental trophoblast (HTR-8/Svneo) cells. HTR-8/Svneo cells were exposed to different concentrations of PFOS (0, 0.01, 0.1, 1.0 mg/L) for 24 h, and the cell survival rates were measured by CCK8. Secretion levels of interleukin-6 (IL-6) , tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10) were detected by ELISA. The mRNA expressions of IL-6, TNF-α and IL-10 were detected by fluorescence quantitative PCR. One-way ANOVA was used to analyse the expressions of inflammatory factors. Compared with the control group, the survival rates of 0.1 and 1.0 mg/L PFOS groups were significantly decreased (<0.05) . Compared with the control group, the secretion levels of IL-6 were decreased in the 0.01, 0.1 and 1.0 mg/L PFOS groups (<0.05) , the concentrations of TNF-α were increased in the 0.01 and 1.0 mg/L PFOS groups (<0.05) , and the concentrations of IL-10 were increased in the 0.1 and 1.0 mg/L PFOS groups (<0.05) . Compared with the control group, the expressions of IL-6 mRNA were increased in the 0.1 and 1.0 mg/L PFOS groups (<0.05) , and the expressions of IL-10 mRNA were decreased in the 0.01 mg/L, 0.1 mg/L and 1.0 mg/L PFOS groups (<0.05) . PFOS can induce changes in the secretion levels of inflammatory cytokines in HTR-8/Svneo cells, resulting in decreased activity of placental trophoblast cells and abnomal placental function.
为研究全氟辛烷磺酸（PFOS）对人胎盘滋养层细胞（HTR-8/Svneo）中炎症因子的影响。将HTR-8/Svneo细胞暴露于不同浓度的PFOS（0、0.01、0.1、1.0 mg/L）中24小时，并用CCK8法检测细胞存活率。采用酶联免疫吸附测定（ELISA）法检测白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的分泌水平。采用荧光定量PCR法检测IL-6、TNF-α和IL-10的mRNA表达。采用单因素方差分析（One-way ANOVA）分析炎症因子的表达情况。与对照组相比，0.1和1.0 mg/L PFOS组的细胞存活率显著降低（<0.05）。与对照组相比，0.01、0.1和1.0 mg/L PFOS组的IL-6分泌水平降低（<0.05），0.01和1.0 mg/L PFOS组的TNF-α浓度升高（<0.05），0.1和1.0 mg/L PFOS组的IL-10浓度升高（<0.05）。与对照组相比，0.1和1.0 mg/L PFOS组的IL-6 mRNA表达升高（<0.05），0.01 mg/L、0.1 mg/L和1.0 mg/L PFOS组的IL-10 mRNA表达降低（<0.05）。PFOS可诱导HTR-8/Svneo细胞中炎症细胞因子分泌水平发生变化，导致胎盘滋养层细胞活性降低及胎盘功能异常。
