Abd-El-Hafez M A, El-Shafee M D, Omar S H, Aburahma A A, Kamar S S
Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Andrology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Folia Morphol (Warsz). 2021;80(3):596-604. doi: 10.5603/FM.a2020.0084. Epub 2020 Aug 4.
Cryptorchidism, one or both hidden testes, is the most common abnormality of male sexual development. Subfertility or infertility is associated with both unilateral and bilateral cryptorchidism. In this study, we investigate the possible ameliorative effect of curcumin (Cur) on the induced-unilateral cryptorchidism testicular injury in both cryptorchid (Cryp) and non-cryptorchid (non-Cryp) scrotal testes through histological, immunohistochemical and morphometrics.
Forty adult male albino rats were divided into: control group, Cur control group, Cryp group, and Cryp+Cur group. The rat model was surgically established by fixing the left testis in the abdomen. The treated groups were subjected to surgically induced-unilateral cryptorchidism on the left side then were given Cur (80 mg/kg) orally, for 20 days. Histological analysis using haematoxylin and eosin and periodic acid Schiff's reaction was done. Immunohistochemistry was performed for proliferating cell nuclear antigen (PCNA); to estimate the proliferation in the germinal epithelium, and vimentin; to evaluate Sertoli cells. The results were confirmed by statistical evaluation of the spermatogenic epithelium height, the seminiferous tubules diameter, the basement membrane thickness, the number of PCNA immunostained cells and the area per cent of vimentin immunostaining.
Distorted seminiferous tubules, substantial degeneration of the germinal epithelium, thickening of the basement membrane with a significant decrease in PCNA and vimentin immunostaining were observed in Cryp group; mainly in the cryptorchid testis. These structural changes were significantly reversed in Crypt+Cur group.
Curcumin proved to be an important and effective medical line for protecting against the unfavourable sequels of cryptorchidism in a rat model.
隐睾症，即一侧或双侧睾丸未降，是男性性发育最常见的异常情况。生育力低下或不育与单侧和双侧隐睾症均有关联。在本研究中，我们通过组织学、免疫组织化学和形态计量学方法，研究姜黄素（Cur）对诱导性单侧隐睾症所致的隐睾（Cryp）和非隐睾（non-Cryp）阴囊睾丸损伤的可能改善作用。
将40只成年雄性白化大鼠分为：对照组、Cur对照组、Cryp组和Cryp+Cur组。通过将左侧睾丸固定于腹腔内手术建立大鼠模型。对治疗组进行左侧手术诱导性单侧隐睾症，然后口服Cur（80mg/kg），持续20天。采用苏木精-伊红染色和过碘酸希夫反应进行组织学分析。对增殖细胞核抗原（PCNA）进行免疫组织化学检测，以评估生精上皮的增殖情况；对波形蛋白进行免疫组织化学检测，以评估支持细胞。通过对生精上皮高度、曲细精管直径、基底膜厚度、PCNA免疫染色细胞数量和波形蛋白免疫染色面积百分比进行统计学评估来证实结果。
在Cryp组中观察到曲细精管扭曲、生精上皮大量变性、基底膜增厚，PCNA和波形蛋白免疫染色显著减少；主要发生在隐睾睾丸中。这些结构变化在Crypt+Cur组中得到显著逆转。
在大鼠模型中，姜黄素被证明是预防隐睾症不良后果的一种重要且有效的药物。
