催乳素在体外和体内对70千道尔顿热休克样信使核糖核酸的调节。
Regulation of 70-kilodalton heat-shock-like messenger ribonucleic acid in vitro and in vivo by prolactin.
作者信息
deToledo S M, Murphy L J, Hatton T H, Friesen H G
机构信息
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
出版信息
Mol Endocrinol. 1987 Jun;1(6):430-4. doi: 10.1210/mend-1-6-430.
A cDNA clone, pNb29, was isolated from a library made from mRNA of Nb2 rat lymphoma cells stimulated by PRL. The nucleotide sequence of pNb29 was found to be identical to the 70-kilodalton heat-shock-like (hsp-70-like) mRNA. The levels of this mRNA increased 8 +/- 3-fold after PRL stimulation of arrested Nb2 cells, and is expressed in different amounts in the normal rat tissues analyzed. To determine whether hsp-70-like mRNA was PRL responsive in vivo, ovine PRL was administered to hypophysectomized rats. A 5 +/- 2-fold increase in hepatic hsp-70-like mRNA was observed 9 h after injection. Thus PRL appears to regulate a heat-shock-like mRNA both in vitro and in vivo. This novel finding extends the already wide range of biological effects ascribed to PRL.
从受催乳素刺激的Nb2大鼠淋巴瘤细胞的mRNA构建的文库中分离出一个cDNA克隆pNb29。发现pNb29的核苷酸序列与70千道尔顿热休克样（hsp - 70样）mRNA相同。在催乳素刺激停滞的Nb2细胞后，这种mRNA的水平增加了8±3倍，并且在所分析的正常大鼠组织中以不同量表达。为了确定hsp - 70样mRNA在体内是否对催乳素作出反应，将绵羊催乳素给予垂体切除的大鼠。注射后9小时观察到肝脏中hsp - 70样mRNA增加了5±2倍。因此，催乳素似乎在体外和体内均调节一种热休克样mRNA。这一新发现扩展了已经归因于催乳素的广泛生物学效应范围。
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