Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, Nigeria.
Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu, Nigeria.
Int J Med Mushrooms. 2020;22(5):467-478. doi: 10.1615/IntJMedMushrooms.2020034776.
The fruiting body of Auricularia auricula-judae has received attention in folk medicine due to its possible medicinal values. Therefore, this study evaluated the immunomodulatory effects of the hot aqueous extract (AAAJ) and the β-D-glucan-rich polysaccharide fraction of A. auricula-judae (BGPA) on specific and nonspecific humoral and cell mediated immune responses in immunocompetent and immunosuppressed mice. Oral supplementation with AAAJ or BGPA (100, 200, or 400 mg/kg) produced significantly high titers of total OVA specific or TT specific IgG1 and IgG2a compared with the levels in untreated control. Oral administration of AAAJ or BGPA (100, 200, or 400 mg/kg) evoked a significant increase in carbon clearance at all doses, indicating stimulation of the reticuloendothelial system, and potentiated the delayed-type hypersensitivity reaction induced by sheep red blood cells (SRBC) compared with the untreated mice. Total lymphocyte count, neutrophil count, and lymphocytes count increased significantly (P < 0.05) at all doses, following acute administration of AAAJ or BGPA (100, 200, or 400 mg/kg), showing increased protection toward cyclophosphamide induced myelosuppression compared with the untreated negative control group. In the hemolytic complement assay, AAAJ and BGPA at all doses significantly (P < 0.05) inhibited the hemolytic activity of the complement proteins on the sensitized SRBC. The present study reveals that the extract holds promise as an immunomodulatory agent and strengthens the rationale for its use in traditional medicine.
银耳的子实体在民间医学中因其可能的药用价值而受到关注。因此，本研究评估了银耳热水提取物（AAAJ）和银耳β-D-葡聚糖丰富的多糖部分（BGPA）对免疫功能正常和免疫抑制小鼠特异性和非特异性体液和细胞介导免疫应答的免疫调节作用。口服补充 AAAJ 或 BGPA（100、200 或 400mg/kg）可显著提高 OVA 特异性或 TT 特异性总 IgG1 和 IgG2a 的滴度，与未处理的对照组相比。口服 AAAJ 或 BGPA（100、200 或 400mg/kg）在所有剂量下均能显著增加碳廓清，表明刺激网状内皮系统，并增强绵羊红细胞（SRBC）诱导的迟发型超敏反应，与未处理的小鼠相比。急性给予 AAAJ 或 BGPA（100、200 或 400mg/kg）后，总淋巴细胞计数、中性粒细胞计数和淋巴细胞计数均显著增加（P<0.05），与未处理的阴性对照组相比，对环磷酰胺诱导的骨髓抑制有更好的保护作用。在溶血补体测定中，AAAJ 和 BGPA 在所有剂量下均显著（P<0.05）抑制了致敏 SRBC 上补体蛋白的溶血活性。本研究表明，该提取物有望成为一种免疫调节剂，并为其在传统医学中的应用提供了更多的依据。
