Moura M C, Procópio T F, Ferreira G R S, Alves R R V, Sá R A, Paiva P M G, Ingmer H, Coelho L C B B, Napoleão T H
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
Centro Acadêmico do Agreste, Universidade Federal de Pernambuco, Caruaru, Pernambuco, Brazil.
J Appl Microbiol. 2021 Mar;130(3):745-754. doi: 10.1111/jam.14811. Epub 2020 Aug 14.
To evaluate the anti-staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) of Myracrodruon urundeuva.
The lectins were evaluated for: effects on growth, aggregation, haemolytic activity and biofilm-forming ability of Staphylococcus aureus clinical isolates nonresistant (8325-4) and multidrug resistant (LAC USA300); interference with the expression of virulence genes (hla, rnaIII and spa) of the Agr system of S. aureus; and synergistic effect with the antibiotics cefoxitin and cefotaxime. MuBL, MuHL and MuLL reduced growth (minimal inhibitory concentration (MIC): 12·5-50 µg ml ) and viability (minimal bactericidal concentration (MBC): 100 µg ml ) of 8325-4 and LAC USA300 cells. MuLL (at ½MIC and MIC) reduced LAC USA300 agglutination. The lectins did not interfere with haemolytic activity and expression of hla, rnaIII and spa genes. Only MuHL was able to reduce the biofilm production by 8325-4 (50-400 µg ml ) and LAC USA300 (400 µg ml ).
The M. urundeuva lectins showed antibacterial activity against nonresistant and resistant clinical isolates of S. aureus and synergistic effects with antibiotics in reducing growth and biofilm formation.
This work reports bioactive molecules capable of acting as anti-staphylococcal agents, since there are increasing reports of multiresistant isolates of this bacterium.
评估从乌伦杜瓦巴西苏木的树皮（MuBL）、心材（MuHL）和树叶（MuLL）中分离得到的凝集素的抗葡萄球菌作用。
对这些凝集素进行了以下评估：对金黄色葡萄球菌临床分离株非耐药株（8325 - 4）和多重耐药株（LAC USA300）的生长、聚集、溶血活性和生物膜形成能力的影响；对金黄色葡萄球菌Agr系统毒力基因（hla、rnaIII和spa）表达的干扰；以及与抗生素头孢西丁和头孢噻肟的协同作用。MuBL、MuHL和MuLL降低了8325 - 4和LAC USA300细胞的生长（最低抑菌浓度（MIC）：12.5 - 50 μg/ml）和活力（最低杀菌浓度（MBC）：100 μg/ml）。MuLL（在1/2 MIC和MIC浓度下）降低了LAC USA300的凝集作用。这些凝集素不干扰溶血活性以及hla、rnaIII和spa基因的表达。只有MuHL能够降低8325 - 4（50 - 400 μg/ml）和LAC USA300（400 μg/ml）的生物膜产生。
乌伦杜瓦巴西苏木凝集素对金黄色葡萄球菌的非耐药和耐药临床分离株显示出抗菌活性，并在降低生长和生物膜形成方面与抗生素具有协同作用。
这项工作报道了能够作为抗葡萄球菌剂的生物活性分子，因为关于这种细菌多重耐药分离株的报道日益增多。
