Physical Chemistry I - Biophysical Chemistry, Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn Street 4a, 44227 Dortmund, Germany.
Institute of Applied Microbiology, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany.
Biochim Biophys Acta Biomembr. 2020 Nov 1;1862(11):183431. doi: 10.1016/j.bbamem.2020.183431. Epub 2020 Aug 1.
Rhamnolipids represent a large class of biologically produced surface-active compounds, which participate in various essential cellular functions. While many studies have reported on the antibacterial and antifungal effects of rhamnolipids, only a few tried to describe the molecular mechanisms underlying these effects. Here, we first review the literature on rhamnolipid-phospholipid interactions and then add own results on a prominent monorhamnolipid congener, RhaCC. By focusing on the interactions between the rhamnolipid and lipid model membranes of different complexity, up to heterogeneous raft-like model biomembranes, we gained new insights into changes of the lateral membrane organization and morphological changes of membrane vesicles induced by partitioning of the rhamnolipid. To this end, AFM, confocal fluorescence microscopy, and Laurdan fluorescence spectroscopy analyses were employed. In summary, we provide a concise description of the physio-chemical effects rhamnolipids impose on lipid membranes, which help us to understand their physiological role.
鼠李糖脂是一大类生物产生的表面活性化合物，参与各种重要的细胞功能。虽然许多研究已经报道了鼠李糖脂的抗菌和抗真菌作用，但只有少数研究试图描述这些作用的分子机制。在这里，我们首先回顾了关于鼠李糖脂-磷脂相互作用的文献，然后添加了我们自己关于一种突出的单鼠李糖脂同系物 RhaCC 的结果。通过关注鼠李糖脂与不同复杂性的脂质模型膜之间的相互作用，直到异质筏状模型生物膜，我们对鼠李糖脂分配引起的侧向膜组织变化和膜泡形态变化有了新的认识。为此，我们采用了原子力显微镜、共聚焦荧光显微镜和 Laurdan 荧光光谱分析。总之，我们对鼠李糖脂对脂质膜施加的物理化学效应进行了简明的描述，这有助于我们理解它们的生理作用。
