Zal Fatemeh, Ahmadi Pardis, Davari Maryam, Khademi Fatemeh, Jahromi Mojgan Akbarzadeh, Anvar Zahra, Jahromi Bahia Namavar
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Reprod Biomed. 2020 Jun 30;18(6):415-424. doi: 10.18502/ijrm.v13i6.7283. eCollection 2020 Jun.
Oxidative stress and GSH-dependent antioxidant system plays a key role in the pathogenesis of polycystic ovary syndrome (PCOS).
We compared glutathione peroxidase (GPx) and glutathione reductase activities and reduced glutathione (GSH) levels in serum and follicular fluid (FF) of the first-retrieved follicle and their impact on quality of oocyte and embryo in PCOS women undergoing IVF.
This cross sectional study was conducted on 80 pairs of blood samples and FF of the first-retrieved follicle from PCOS women, at the Infertility center of Ghadir Mother and Child Hospital. The mean activity of GPx and GR, also GSH levels in the serum and FF were compared to the quality of the first follicle and resultant embryo.
Retrieved oocytes included 53 (66.25%) MII, 17 (21.25%) MI, and 10 (12.5%) germinal vesicles; after IVF 42 (52.50%) embryos with grade I and 11 (13.75%) with grade II were produced. The mean values for all three antioxidants were higher in the FF compared to serum (p 0.001). Also all of the mean measured levels were significantly higher in the FF of the MII oocytes compared to that of oocytes with lower grades (p = 0.012, 0.006 and 0.012, respectively). The mean GPX activity and GSH levels were significantly higher in the serum (p = 0.016 and 0.012, respectively) and FF (p = 0.001 for both) of the high-quality grade I embryos.
GSH-dependent antioxidant system functions more efficiently in the FF of oocytes and embryos with higher quality.
氧化应激和谷胱甘肽（GSH）依赖的抗氧化系统在多囊卵巢综合征（PCOS）的发病机制中起关键作用。
我们比较了接受体外受精（IVF）的PCOS女性首次获取卵泡的血清和卵泡液（FF）中谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶活性以及还原型谷胱甘肽（GSH）水平，及其对卵母细胞和胚胎质量的影响。
本横断面研究在加迪尔母婴医院不孕不育中心对80对PCOS女性首次获取卵泡的血样和FF进行。将血清和FF中GPx和GR的平均活性以及GSH水平与首个卵泡和所得胚胎的质量进行比较。
获取的卵母细胞包括53个（66.25%）处于第二次减数分裂中期（MII）、17个（21.25%）处于第一次减数分裂中期（MI）和10个（12.5%）处于生发泡期；IVF后产生了42个（52.50%）I级胚胎和11个（13.75%）II级胚胎。与血清相比，FF中所有三种抗氧化剂的平均值更高（p < 0.001）。此外，与较低等级卵母细胞相比，MII卵母细胞FF中所有测量水平的平均值均显著更高（分别为p = 0.012、0.006和0.012）。高质量I级胚胎的血清（分别为p = 0.016和0.012）和FF（两者均为p = 0.001）中GPX活性和GSH水平的平均值显著更高。
GSH依赖的抗氧化系统在质量较高的卵母细胞和胚胎的FF中功能更有效。
