Long M W, Heffner C H
Pediatric Hematology/Oncology Division, University of Michigan, Ann Arbor 48109-0684.
Exp Hematol. 1988 Jan;16(1):62-70.
Many studies report the presence of platelet-megakaryocyte-associated antigens on human immature and mature megakaryocytes as well as progenitor cells of this lineage. However, studies of purified megakaryocytes or megakaryocyte colonies yield little information on the effect(s) of growth factors in regulating antigenic expression, the kinetics of expression, or the relative content of antigens expressed. We report the detection of human megakaryocyte antigens using a solid-phase radioimmunoassay. The assay is linear, specific, and detects a range of approximately 300-10,000 megakaryocytes per aliquot. Analyses of unstimulated and stimulated suspension cultures indicate that activities known to influence megakaryocyte development in vitro also increase total megakaryocyte antigenic content. Additionally, studies on human erythroleukemia cells show that those cells constitutively express megakaryocyte antigens and increase these antigens when stimulated. Finally, since the specificity of the assay is that of the primary antibody, this procedure is easily extended to analysis of antigenic expression in other lineages.
许多研究报告称，在人类未成熟和成熟的巨核细胞以及该谱系的祖细胞上存在血小板 - 巨核细胞相关抗原。然而，对纯化的巨核细胞或巨核细胞集落的研究，关于生长因子在调节抗原表达、表达动力学或所表达抗原的相对含量方面的作用，提供的信息很少。我们报告了使用固相放射免疫测定法检测人类巨核细胞抗原。该测定法呈线性、具有特异性，每份样品可检测约300 - 10,000个巨核细胞。对未刺激和刺激后的悬浮培养物的分析表明，已知在体外影响巨核细胞发育的活性物质也会增加总巨核细胞抗原含量。此外，对人类红白血病细胞的研究表明，这些细胞组成性表达巨核细胞抗原，并在受到刺激时增加这些抗原的表达。最后，由于该测定法的特异性取决于一抗，此方法可轻松扩展到其他谱系抗原表达的分析。
