Matsuzaki G, Yoshikai Y, Kishihara K, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
J Immunol. 1988 Jan 15;140(2):384-7.
Sequential appearance of the expression of T cell antigen receptor genes was investigated in the thymus of irradiated mice at the early stage after transplantation of Thy-1 congeneic H-2 compatible allogeneic bone marrow cells. The first cells to repopulate the thymus on day 7 after bone marrow transplantation were intrathymic radioresistant T cell precursors, which expanded mainly to CD4+CD8+ host-type thymocytes by day 14. A high level of gamma gene expression but a much reduced level of alpha and beta gene expression were detected in the host-type thymocytes on day 7. During regeneration of these cells, gamma-chain messages fell to low level and alpha and beta mRNA levels increased. The thymus of the recipients began to be repopulated by donor-derived T cells about 2 wk after bone marrow transplantation and was almost completely replaced by the third week. An ordered expression of gamma then beta and alpha-chain gene transcript was also observed in the donor-type thymocytes at the early stage after bone marrow transplantation. The use of thymocytes at early stage in whole-body irradiated bone marrow chimera provides a pertinent source for investigating the molecular mechanism of T cell differentiation in adult thymus.
在移植了Thy-1同基因H-2相容的同种异体骨髓细胞后的早期，研究了受照射小鼠胸腺中T细胞抗原受体基因表达的顺序出现情况。骨髓移植后第7天首次重新填充胸腺的细胞是胸腺内抗辐射的T细胞前体，到第14天，这些前体主要扩增为CD4+CD8+宿主型胸腺细胞。在第7天的宿主型胸腺细胞中检测到高水平的γ基因表达，但α和β基因表达水平大幅降低。在这些细胞的再生过程中，γ链信息降至低水平，α和β mRNA水平升高。骨髓移植后约2周，受体的胸腺开始被供体来源的T细胞重新填充，到第三周时几乎完全被取代。在骨髓移植后的早期，在供体型胸腺细胞中也观察到γ然后β和α链基因转录本的有序表达。在全身照射的骨髓嵌合体中早期使用胸腺细胞为研究成年胸腺中T细胞分化的分子机制提供了一个相关来源。
