Lau J L, Fowler J E, Ghosh L
Division of Urology, University of Illinois College of Medicine, Chicago 60680.
J Urol. 1988 Jan;139(1):170-5. doi: 10.1016/s0022-5347(17)42347-0.
Epidermal growth factor (EGF) is a cell-regulating polypeptide that appears important to the maintenance and function of some benign tissues and to the transformation and proliferation of certain malignancies. In humans the highest concentrations of EGF are found in the urine. We investigated possible interactions between EGF and normal and neoplastic tissues of the urinary system with indirect immunohistochemical staining of paraffin-embedded tissue sections. A polyclonal antibody directed against mouse EGF but shown to react with human EGF was used in the assays. Positive staining was granular in nature and confined to the cytoplasm. Staining of the renal parenchyma (N = 5) was observed in the epithelium of the proximal and distal tubules and the collecting ducts. There was staining of clear cell (N = 6) and papillary (N = 3) carcinomas of the kidney. Staining of the normal urothelium (N = 5) was limited to superficial cells. All transitional cell (N = 21) and squamous (N = 2) carcinomas of the bladder stained. Subjectively, the staining intensity of the transitional cell carcinomas correlated inversely with tumor differentiation. In light of evidence that internalized, receptor-bound EGF is rapidly degraded, the striking immunohistochemical demonstration of cytoplasmic EGF suggests active synthesis. EGF synthesized by urothelial and renal carcinomas may be involved in an autocrine mechanism of malignant proliferation.
表皮生长因子（EGF）是一种细胞调节多肽，对某些良性组织的维持和功能以及某些恶性肿瘤的转化和增殖似乎很重要。在人类中，尿液中EGF的浓度最高。我们通过对石蜡包埋组织切片进行间接免疫组织化学染色，研究了EGF与泌尿系统正常组织和肿瘤组织之间可能的相互作用。检测中使用了一种针对小鼠EGF但已证明可与人EGF反应的多克隆抗体。阳性染色本质上呈颗粒状，局限于细胞质。在近端和远端小管以及集合管的上皮中观察到肾实质（N = 5）的染色。肾透明细胞癌（N = 6）和乳头状癌（N = 3）有染色。正常尿路上皮（N = 5）的染色仅限于表层细胞。膀胱的所有移行细胞癌（N = 21）和鳞状细胞癌（N = 2）均有染色。主观上，移行细胞癌的染色强度与肿瘤分化呈负相关。鉴于有证据表明内化的、与受体结合的EGF会迅速降解，细胞质EGF显著的免疫组织化学表现提示有活跃的合成。尿路上皮癌和肾癌合成的EGF可能参与了恶性增殖的自分泌机制。
