Xiong Lin, Tan Wan-long, Yu Zhao-cun, Wu Yuan-dong, Huang He, Zhao Guo-zhi, Zhu Wen-hui, Zheng Shao-bin
Department of Urinary Surgery, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jun;26(6):805-9.
To detect the expression of apoptosis gene PDCD5 in tissues of normal human kidney, renal clear cell carcinoma, normal bladder and bladder carcinoma, and explore the role of PDCD5 gene in renal clear cell carcinoma and bladder carcinoma.
Indirect immunohistochemistry was employed to detect PDCD5 expression in 63 kidney specimens and 42 bladder specimens. Positive expression rates and intensity of PDCD5 protein expression in the kidney tissue were investigated microscopically and by computerized image analysis. Positive expression rate in the bladder tissue was investigated by microscopic observation.
The results of immunohistochemical staining showed PDCD5 protein overexpression in the renal tubule of normal human kidney tissues and downregulation with the stage increase of renal clear cell carcinoma. PDCD5 protein expression showed statistical significance in tissues of normal kidney and renal clear cell carcinoma in all stages. No obvious PDCD5 expression was detected in the tissues of normal human bladder and bladder carcinoma.
PDCD5 is an important apoptosis-regulating factor in the occurrence of renal clear cell carcinoma, and its expression is extremely low in tissues of normal human bladder and bladder carcinoma.
检测凋亡基因PDCD5在正常人肾组织、肾透明细胞癌组织、正常膀胱组织及膀胱癌组织中的表达情况，探讨PDCD5基因在肾透明细胞癌和膀胱癌中的作用。
采用间接免疫组织化学法检测63例肾脏标本和42例膀胱标本中PDCD5的表达。通过显微镜观察及计算机图像分析，研究肾组织中PDCD5蛋白表达的阳性率及强度。通过显微镜观察研究膀胱组织中的阳性表达率。
免疫组织化学染色结果显示，PDCD5蛋白在正常人肾组织肾小管中呈过表达，且随肾透明细胞癌分期增加而表达下调。PDCD5蛋白表达在正常肾组织和各期肾透明细胞癌组织中具有统计学意义。在正常人膀胱组织和膀胱癌组织中未检测到明显的PDCD5表达。
PDCD5是肾透明细胞癌发生过程中的重要凋亡调节因子，其在正常人膀胱组织和膀胱癌组织中的表达极低。
