SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, 603 203, Chennai, Tamil Nadu, India.
Lab PCN 206, Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, 226 031, Uttar Pradesh, India.
Fish Shellfish Immunol. 2020 Nov;106:332-340. doi: 10.1016/j.fsi.2020.07.068. Epub 2020 Aug 3.
The occurrences of multiple drug-resistant strains have been relentlessly increasing in recent years. The aquaculture industry has encountered major disease outbreaks and crucially affected by this situation. The usage of non-specific chemicals and antibiotics expedites the stimulation of resistant strains. Triggering the natural defense mechanism would provide an effective and safest way of protecting the host system. Hence, we have investigated the innate immune function of serine/threonine-protein kinase (STPK) in Macrobrachium rosenbergii (Mr). The in-silico protein analysis resulted in the identification of cationic antimicrobial peptide, MrSL-19, with interesting properties from STPK of M. rosenbergii. Antimicrobial assay, FACS and SEM analysis demonstrated that the peptide potentially inhibits Staphylococcus aureus by interacting with its membrane. The toxic study on MrSL-19 demonstrated that the peptide is not toxic against HEK293 cells as well as human erythrocytes. This investigation showed the significant innate immune property of an efficient cationic antimicrobial peptide, MrSL-19 of STPK from M. rosenbergii.
近年来，多种耐药菌株的出现率不断上升。水产养殖业遭遇了重大疾病爆发，并受到了这一情况的严重影响。非特异性化学物质和抗生素的使用加速了耐药菌株的刺激。触发自然防御机制将为保护宿主系统提供一种有效且最安全的方式。因此，我们研究了罗氏沼虾丝氨酸/苏氨酸蛋白激酶 (STPK) 的先天免疫功能。通过对罗氏沼虾 STPK 的计算机蛋白分析，发现了具有有趣特性的阳离子抗菌肽 MrSL-19。抗菌测定、FACS 和 SEM 分析表明，该肽通过与细胞膜相互作用可能抑制金黄色葡萄球菌。对 MrSL-19 的毒性研究表明，该肽对 HEK293 细胞和人红细胞没有毒性。这项研究表明，罗氏沼虾 STPK 的高效阳离子抗菌肽 MrSL-19 具有显著的先天免疫特性。
