实验性自身免疫性心肌炎Lewis大鼠心脏中骨膜蛋白的免疫组织化学分析
Immunohistochemical analysis of periostin in the hearts of Lewis rats with experimental autoimmune myocarditis.
作者信息
Choi Yuna, Oh Hanseul, Ahn Meejung, Kang Taeyoung, Chun Jiyoon, Shin Taekyun, Kim Jeongtae
机构信息
College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea.
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
出版信息
J Vet Med Sci. 2020 Oct 30;82(10):1545-1550. doi: 10.1292/jvms.20-0225. Epub 2020 Aug 5.
Periostin plays a critical role in tissue regeneration and homeostasis. The aim of this study was to evaluate the changes in periostin levels in the hearts of rats with experimental autoimmune myocarditis (EAM). Western blot analysis revealed that the expression levels of periostin and alpha-smooth muscle actin were significantly increased at day 14 post-immunization. Immunohistochemical analysis indicated that periostin was expressed in macrophages and fibroblasts in the hearts of EAM-induced rats. In conclusion, these results suggest that increased periostin expression in macrophages and fibroblasts promotes cardiac fibrosis in EAM-induced rats, potentially by enhancing immune cell infiltration. Therefore, periostin should be further investigated as a candidate therapeutic target for myocarditis.
骨膜蛋白在组织再生和内环境稳定中起关键作用。本研究旨在评估实验性自身免疫性心肌炎（EAM）大鼠心脏中骨膜蛋白水平的变化。蛋白质免疫印迹分析显示，免疫后第14天骨膜蛋白和α-平滑肌肌动蛋白的表达水平显著升高。免疫组织化学分析表明，骨膜蛋白在EAM诱导大鼠心脏的巨噬细胞和成纤维细胞中表达。总之，这些结果表明，巨噬细胞和成纤维细胞中骨膜蛋白表达增加可能通过增强免疫细胞浸润促进EAM诱导大鼠的心脏纤维化。因此，应进一步研究骨膜蛋白作为心肌炎候选治疗靶点的可能性。
Zotero 插件