新鲜视网膜母细胞瘤细胞在绒毛尿囊膜上的成功生长。

Successful growth of fresh retinoblastoma cells in chorioallantoic membrane.

作者信息

Fialho Silvia L, Silvestrini Bárbara R, Vieira Juliana, Paiva Mayara R B, Silva Luciana M, Chahud Fernando, Silva-Cunha Armando, Correa Zelia M, Jorge Rodrigo

机构信息

Pharmaceutical Research and Development, Ezequiel Dias Foundation, Belo Horizonte, Brazil.

Department of Ophthalmology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.

出版信息

Int J Retina Vitreous. 2020 Jul 29;6:33. doi: 10.1186/s40942-020-00236-x. eCollection 2020.

DOI:10.1186/s40942-020-00236-x

PMID:32760596

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7391810/

Abstract

The authors developed a retinoblastoma model using fresh harvested cells from an enucleated eye that were transplanted in chick embryos (chorioallantoic membrane model). The transplanted embryos were treated with escalating doses of Melphalan. This exploratory model was developed with the goal of testing drug sensitivity. Our findings suggest this tumor model could be employed to personalize treatment for patients with retinoblastoma, especially those with bilateral and more refractory disease.

摘要

作者使用从摘除眼球新鲜获取的细胞建立了视网膜母细胞瘤模型，这些细胞被移植到鸡胚中（绒毛尿囊膜模型）。对移植的胚胎给予递增剂量的美法仑治疗。开发这个探索性模型的目的是测试药物敏感性。我们的研究结果表明，这种肿瘤模型可用于为视网膜母细胞瘤患者制定个性化治疗方案，尤其是那些患有双侧及更难治性疾病的患者。