He Haifeng, Meng Xiangying, Deng Lili, Sun Qi, Huang Xuelong, Lan Ning, Zhao Feng
Jiangxi Engineering Laboratory of Waterborne Coating, School of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China.
Key Laboratory for Green Chemical Process of Ministry of Education and School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan, 430205, PR China.
Org Biomol Chem. 2020 Aug 19;18(32):6357-6363. doi: 10.1039/d0ob01396j.
The development of a novel near-infrared (NIR) probe for the detection of toxic Hg2+ in organisms with high selectivity and sensitivity is of great interest but remains a great challenge. Hence, in this work, a new NIR fluorescence enhanced sensor (TBBA), which contains a D-A structure as the NIR fluorophore and rhodanine-3-acetic acid as the receptor, has been developed for the detection of Hg2+ with high selectivity, sensitivity, low limit of detection (13.10 nM) and good binding constant (2.37 × 104 M-1). The mechanism of TBBA response to Hg2+ was further proved by 1H NMR titration, HRMS, and theoretical calculations. Furthermore, TBBA is applied as a fluorescent probe for imaging living cells and zebrafish, indicating that it can be potentially applied for Hg2+ sensing in both environmental and biology fields.
开发一种用于高选择性和高灵敏度检测生物体内有毒Hg2+的新型近红外（NIR）探针备受关注，但仍然是一个巨大的挑战。因此，在这项工作中，一种新的近红外荧光增强传感器（TBBA）被开发出来，它包含一个作为近红外荧光团的D-A结构和作为受体的罗丹宁-3-乙酸，用于高选择性、高灵敏度地检测Hg2+，其检测限低（13.10 nM）且结合常数良好（2.37×104 M-1）。1H NMR滴定、高分辨质谱（HRMS）和理论计算进一步证明了TBBA对Hg2+的响应机制。此外，TBBA被用作活细胞和斑马鱼成像的荧光探针，表明它在环境和生物领域都有潜在的Hg2+传感应用。
