D-半乳糖胺处理的大鼠肝脏中肝细胞增殖刺激因子的部分纯化及特性鉴定

Partial purification and characterization of hepatocyte proliferation stimulatory factor from liver of rats treated with D-galactosamine.

作者信息

Suemori S, Eto T, Yamada K, Nakamura T, Nakanishi T, Kajiyama G

机构信息

Department of Internal Medicine, Hiroshima University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Jan 15;150(1):133-9. doi: 10.1016/0006-291x(88)90496-2.

DOI:10.1016/0006-291x(88)90496-2

PMID:3276307

Abstract

The partial purification and characterization of a hepatocyte proliferation stimulatory factor (HPSF) isolated from the liver of D-galactosamine-treated rats are described. The HPSF was a heat-labile, acid-stable and trypsin-sensitive protein. The partially purified HPSF stimulated DNA synthesis and increased the labeling index of parenchymal hepatocytes at 5 micrograms/ml and maximally at 50 micrograms/ml. The effect of HPSF in stimulating DNA synthesis was synergistic with that of insulin plus epidermal growth factor (EGF). The HPSF was scarcely detected in normal rat liver. The results obtained indicate that this HPSF is distinct from insulin, multiplication-stimulating activity (MSA), EGF and other hepatocyte growth factors previously reported, and suggest a plausible role for HPSF in the regeneration of liver tissue following hepatotoxic damage.

摘要

本文描述了从经D-半乳糖胺处理的大鼠肝脏中分离出的肝细胞增殖刺激因子（HPSF）的部分纯化及特性鉴定。HPSF是一种对热不稳定、对酸稳定且对胰蛋白酶敏感的蛋白质。部分纯化的HPSF在浓度为5微克/毫升时刺激DNA合成，并提高实质肝细胞的标记指数，在50微克/毫升时达到最大刺激效果。HPSF刺激DNA合成的作用与胰岛素加表皮生长因子（EGF）具有协同性。在正常大鼠肝脏中几乎检测不到HPSF。所得结果表明，这种HPSF与胰岛素、增殖刺激活性（MSA）、EGF及先前报道的其他肝细胞生长因子不同，并提示HPSF在肝毒性损伤后肝组织再生中可能发挥作用。