Grapengiesser E, Gylfe E, Hellman B
Department of Medical Cell Biology, Uppsala University, Sweden.
Biochem Biophys Res Commun. 1988 Jan 15;150(1):419-25. doi: 10.1016/0006-291x(88)90537-2.
The cytoplasmic Ca2+ concentration (Ca2+i) was measured in single pancreatic beta-cells from ob/ob-mice using the fluorescent indicator fura-2. Raising the glucose concentration from 3 to 20 mM resulted in 25% initial lowering of Ca2+i, followed by 250% rise above the basal level of 49 +/- 3 nM. Tolbutamide (100 microM) was as effective as glucose in increasing Ca2+i, although its action was more rapid and not preceded by any reduction. The results support the concept that stimulated removal of Ca2+ from the cytoplasm is an essential part of the physiological glucose effect on the pancreatic beta-cells.
使用荧光指示剂fura-2测量ob/ob小鼠单个胰岛β细胞中的细胞质Ca2+浓度（Ca2+i）。将葡萄糖浓度从3 mM提高到20 mM会导致Ca2+i最初降低25%，随后在49±3 nM的基础水平之上升高250%。甲苯磺丁脲（100 μM）在增加Ca2+i方面与葡萄糖一样有效，尽管其作用更快，且在升高之前没有任何降低。这些结果支持了这样一种观点，即从细胞质中刺激去除Ca2+是生理葡萄糖对胰岛β细胞作用的一个重要部分。
