Arkhammar P, Nilsson T, Berggren P O
Department of Medical Cell Biology, University of Uppsala, Sweden.
Biochem Biophys Res Commun. 1989 Feb 28;159(1):223-8. doi: 10.1016/0006-291x(89)92426-1.
Fura-2 loaded pancreatic beta-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high concentration of the sugar. Probenecid both reduced basal efflux of fura-2 and prevented that promoted by glucose. There was no interference of the drug with glucose-induced either insulin release or rise in [Ca2+]i. When applying fura-2 in pancreatic beta-cells, the use of probenecid markedly improves the measurements of [Ca2+]i.
研究了从肥胖高血糖小鼠分离出的负载有Fura-2的胰腺β细胞的细胞质游离钙离子浓度（[Ca2+]i）、胰岛素释放及指示剂流出情况。在无葡萄糖情况下，Fura-2持续流出，高浓度糖刺激可使其显著增加。丙磺舒既降低了Fura-2的基础流出量，又阻止了葡萄糖促进的流出。该药物对葡萄糖诱导的胰岛素释放或[Ca2+]i升高无干扰。在胰腺β细胞中应用Fura-2时，丙磺舒的使用显著改善了[Ca2+]i的测量。
