Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China.
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China.
Int J Biol Macromol. 2020 Dec 1;164:2788-2794. doi: 10.1016/j.ijbiomac.2020.08.012. Epub 2020 Aug 4.
The transcription factor JunB can induce physiological or pathological responses to various stimuli, including immune stimulants and bacteria, and plays an important role in the immune response process. In this study, we identified a JunB family member in Schizothorax prenanti (S. prenanti), which was designated SpJunB. The complete coding sequence (CDS) of SpJunB was 930 bp in length, which was submitted to GenBank (ID: MN215886). SpJunB encodes a putative protein of 309 amino acids, which is highly homologous to JunB of common carp. The SpJunB protein contained a conserved JunB domain, and its 3D structure was also highly similar to (77.61%) the human SpJunB protein. SpJunB was found to be extensively expressed in various tissues, with the highest expression in the spleen. The expression of SpJunB was significantly upregulated after Aeromonas hydrophila (A. hydrophila) challenge. Prokaryotic expression indicated that a 51 kDa recombinant protein was obtained after induction with 1.5 mmol/L isopropyl-beta-D-thiogalactopyranoside (IPTG) for 6 h at 37 °C. The expression levels of IL-1β, IL-6 and IL-8 were significantly upregulated (p < 0.01) after treatment of S. prenanti with the SpJunB protein. The activities of SOD, AKP and LZM were also significantly increased (p < 0.01) after the treatment of S. prenanti with the SpJunB protein. Simultaneously, the SpJunB protein reduced the infection rate of A. hydrophila in S. prenanti. In conclusion, SpJunB may improve the immune functions of S. prenanti. It will be beneficial to further study the immune mechanism of JunB in fish.
转录因子 JunB 可以诱导对各种刺激的生理或病理反应，包括免疫刺激剂和细菌，并在免疫反应过程中发挥重要作用。本研究在齐口裂腹鱼（Schizothorax prenanti）中鉴定了一个 JunB 家族成员，命名为 SpJunB。SpJunB 的完整编码序列（CDS）长 930bp，已提交至 GenBank（ID：MN215886）。SpJunB 编码一个推测的 309 个氨基酸的蛋白质，与鲤鱼的 JunB 高度同源。SpJunB 蛋白含有一个保守的 JunB 结构域，其 3D 结构与（77.61%）人类 SpJunB 蛋白也高度相似。SpJunB 在各种组织中广泛表达，在脾脏中的表达最高。在受到嗜水气单胞菌（Aeromonas hydrophila）挑战后，SpJunB 的表达显著上调。原核表达表明，在 37°C 下用 1.5mmol/L 异丙基-β-D-硫代半乳糖吡喃糖苷（IPTG）诱导 6 小时后，可获得 51kDa 的重组蛋白。用 SpJunB 蛋白处理齐口裂腹鱼后，IL-1β、IL-6 和 IL-8 的表达水平显著上调（p<0.01）。SpJunB 蛋白处理后，SOD、AKP 和 LZM 的活性也显著升高（p<0.01）。同时，SpJunB 蛋白降低了齐口裂腹鱼感染嗜水气单胞菌的比率。综上所述，SpJunB 可能改善了齐口裂腹鱼的免疫功能。这将有助于进一步研究 JunB 在鱼类中的免疫机制。
