Cranston D, Wood K J, Morris P J
Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, UK.
Br J Surg. 1988 Jan;75(1):18-22. doi: 10.1002/bjs.1800750108.
The effect of splenectomy on renal allograft survival is not clear. In the rat, spleens isolated from recipients with functioning grafts have been shown to be a major source of cells that are capable of suppressing the rejection response (suppressor T lymphocytes). Thus the removal of the spleen in these allograft recipients could be detrimental to renal allograft survival. This study investigates this hypothesis, and looks for the presence of suppressor cells in other lymphoid organs apart from the spleen. In the rat renal allograft model, donor Lewis spleen cells given to DA recipients intravenously 1 week before transplantation of a Lewis kidney leads to indefinite allograft survival (median survival time (MST) greater than 100 days). Splenectomy before or after pretreatment with donor spleen cells failed to abrogate this effect (MST greater than 100 days). Experiments were performed in which cells or serum were prepared from long-term surviving splenectomized animals which had already been pretreated and transplanted, and then were injected into untreated recipients (adoptive transfer experiments). This was done to determine if cells capable of suppressing graft rejection were present in lymphoid organs outside the spleen in these splenectomized recipients. Thus the IV transfer of 10(8) lymph node cells harvested from splenectomized DA recipients with a long-term surviving LEW graft (LTS), into untreated but lightly irradiated (200 rad) DA recipients resulted in indefinite survival of a fresh Lewis kidney (MST greater than 100 days). In contrast, adoptive transfer of normal DA lymph node cells was ineffective (MST 13 days). Thus splenectomy is not necessarily detrimental to graft survival, as cells capable of preventing graft rejection are found in other lymphoid organs, such as lymph nodes, in splenectomized recipients.
脾切除术对肾移植存活的影响尚不清楚。在大鼠中，已证明从具有功能正常移植物的受体分离出的脾脏是能够抑制排斥反应的细胞（抑制性T淋巴细胞）的主要来源。因此，在这些同种异体移植受体中切除脾脏可能对肾移植存活不利。本研究对这一假设进行了调查，并寻找除脾脏外其他淋巴器官中抑制细胞的存在。在大鼠肾移植模型中，在移植Lewis肾前1周给DA受体静脉注射供体Lewis脾细胞可导致同种异体移植物无限期存活（中位存活时间（MST）大于100天）。在供体脾细胞预处理之前或之后进行脾切除术并不能消除这种效果（MST大于100天）。进行了实验，从已经接受预处理并移植的长期存活的脾切除动物中制备细胞或血清，然后注射到未处理的受体中（过继转移实验）。这样做是为了确定在这些脾切除受体的脾脏外的淋巴器官中是否存在能够抑制移植物排斥的细胞。因此，将从具有长期存活LEW移植物（LTS）的脾切除DA受体收获的10(8)个淋巴结细胞静脉内转移到未处理但轻度照射（200拉德）的DA受体中，可导致新鲜Lewis肾无限期存活（MST大于100天）。相比之下，正常DA淋巴结细胞的过继转移无效（MST为13天）。因此，脾切除术不一定对移植物存活不利，因为在脾切除受体的其他淋巴器官如淋巴结中发现了能够防止移植物排斥的细胞。
