Cohen Zoya, Maimon Yair, Berger Raanan, Morag Ofir, Golan Talia, Samuels Noah
Tal Center for Integrative Medicine, Institute of Oncology, Sheba Medical Center, Ramat Gan 52621, Israel.
Institute of Oncology, Sheba Medical Center, Ramat Gan 52621, Israel.
Biomed Rep. 2020 Sep;13(3):17. doi: 10.3892/br.2020.1323. Epub 2020 Jul 2.
Innate immunity serves an important role in the healthy population, providing surveillance and protection against infections. Chemotherapy suppresses the body's immune system, including neutrophil and natural killer (NK) cell numbers and activity. This leads to an increased risk of infection which often requires the reduction or even discontinuation of the chemotherapeutic regimens. The botanical formula LCS102 was designed to stimulate the body's immune system. The effect of the formula on innate immunity was examined in human blood samples, as were its effect on the anti-cancer activity of chemotherapeutic agents on human cancer cells. Blood samples drawn from 20 volunteers (19 healthy subjects; 1 patient with breast cancer undergoing chemotherapy) and were exposed to LCS102. The effects on neutrophil and NK cell activity were tested using FACS. The anti-cancer effects of LCS102 were tested on T24, A549, MCF7, PANC-1 and U2OS human cancer cell lines, as were the effects of the formula on doxorubicin, Taxol, etoposide and cisplatin-treated cells using a sulforodamine B viability assay. LCS102 was shown to significantly increase the percentage of activated neutrophils and NK cells in the blood samples tested. The formula did not inhibit the cytotoxic effects of the chemotherapeutic agents, and in certain cases increased their anti-cancer activity. Further research is required to improve our understanding of the clinical value of LCS102; however, it may serve as an adjuvant during chemotherapy, to reduce the effects of chemotherapy on innate immunity.
固有免疫在健康人群中发挥着重要作用，提供对感染的监测和保护。化疗会抑制人体免疫系统，包括中性粒细胞和自然杀伤（NK）细胞的数量及活性。这会导致感染风险增加，常常需要减少甚至中断化疗方案。植物配方LCS102旨在刺激人体免疫系统。该配方对固有免疫的影响在人体血液样本中进行了检测，其对化疗药物对人类癌细胞的抗癌活性的影响也进行了检测。从20名志愿者（19名健康受试者；1名正在接受化疗的乳腺癌患者）采集血液样本并使其接触LCS102。使用流式细胞术检测对中性粒细胞和NK细胞活性的影响。LCS102对T24、A549、MCF7、PANC - 1和U2OS人类癌细胞系的抗癌作用进行了检测，该配方对阿霉素、紫杉醇、依托泊苷和顺铂处理的细胞的影响也使用磺酰罗丹明B活力测定法进行了检测。结果显示，LCS102能显著增加所检测血液样本中活化中性粒细胞和NK细胞的百分比。该配方并未抑制化疗药物的细胞毒性作用，在某些情况下还增强了它们的抗癌活性。需要进一步研究以加深我们对LCS102临床价值的理解；然而，它可能在化疗期间作为佐剂，以减轻化疗对固有免疫的影响。
