Wan Daniel, Wang Xiaowei, Nakamura Ryosuke, Alcocer Marcos J C, Falcone Franco H
University of Nottingham, School of Pharmacy, Nottingham, England, UK.
School of Biosciences, University of Nottingham, Nottingham, UK.
Methods Mol Biol. 2020;2163:145-153. doi: 10.1007/978-1-0716-0696-4_11.
Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.
测定人血样本中过敏原特异性免疫球蛋白E（IgE）水平是评估过敏致敏反应的一项重要诊断技术。人血清样本中特异性IgE的存在可通过用稀释血清致敏人源化大鼠嗜碱性粒细胞白血病（RBL）细胞系，并在受到疑似过敏原攻击后测量细胞活化来进行检测。传统上，这是通过测量RBL脱颗粒时释放的β-己糖胺酶水平来实现的。在此，我们描述了两种最近开发的人源化RBL报告细胞系的应用，它们具有更高的灵敏度，适用于高通量实验。
