嗜碱性粒细胞激活技术：外化颗粒基质的染色
Basophil Activation Techniques: Staining of Exteriorized Granule Matrix.
作者信息
Elst Jessy, Sabato Vito, Hagendorens Margo M, van Houdt Michel, Faber Margaretha A, Bridts Chris H, Ebo Didier G, Mertens Christel
机构信息
Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, University of Antwerp, Antwerp University Hospital, Antwerp, Belgium.
Department of Immunology and Allergology, AZ Jan Palfijn Gent, Gent, Belgium.
出版信息
Methods Mol Biol. 2020;2163:213-218. doi: 10.1007/978-1-0716-0696-4_17.
The basis of traditional flow cytometry allergy diagnosis is measurement of the expression of basophilic surface activation and/or degranulation markers. Basophils, upon encounter with a specific allergen that cross-links surface FcRI-bound IgE antibodies, not only secrete and release quantifiable bioactive mediators but also upregulate the expression of different markers (e.g., CD63, CD203c) which can be detected by multicolor flow cytometry using specific monoclonal antibodies. Here, we describe a novel technique that relies upon the staining of exteriorized anionic proteoglycans from a basophil granule matrix by cationic fluorescent avidin probes.
传统流式细胞术过敏诊断的基础是测量嗜碱性粒细胞表面活化和/或脱颗粒标志物的表达。嗜碱性粒细胞在遇到与表面FcRI结合的IgE抗体发生交联的特定过敏原时，不仅会分泌和释放可量化的生物活性介质，还会上调不同标志物（如CD63、CD203c）的表达，这些标志物可通过使用特异性单克隆抗体的多色流式细胞术进行检测。在此，我们描述了一种新技术，该技术依赖于用阳离子荧光抗生物素蛋白探针染色来自嗜碱性粒细胞颗粒基质的外化阴离子蛋白聚糖。
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