人肥大细胞和嗜碱性粒细胞上与Mas相关的G蛋白偶联受体MRGPRX2的测量与功能分析
Measurement and Functional Analysis of the Mas-Related G Protein-Coupled Receptor MRGPRX2 on Human Mast Cells and Basophils.
作者信息
Elst Jessy, Sabato Vito, Hagendorens Margo M, van Houdt Michel, Faber Margaretha A, Bridts Chris H, Ebo Didier G, Mertens Christel
机构信息
Faculty of Medicine and Health Science, Department of Immunology, Allergology, Rheumatology and the Infla-Med Centre of Excellence, University of Antwerp, Antwerp University Hospital, Antwerp, Belgium.
Department of Immunology and Allergology, AZ Jan Palfijn Gent, Ghent, Belgium.
出版信息
Methods Mol Biol. 2020;2163:219-226. doi: 10.1007/978-1-0716-0696-4_18.
Basophils and mast cells (MCs) are important effector cells in the immune system. For a long time, it has been known that these cells can be activated though the cross-linking of IgE antibodies bound to their high-affinity receptor (FcεRI). However, evidence has accumulated suggesting that these cells can also be activated by various IgE-independent mechanisms. Occupation of MAS Related GPR Family Member X2 (MRGPRX2), a G protein-coupled receptor, is described as an alternative IgE-independent activation mechanism. Here we describe a flow cytometric technique to analyze MRGPRX2 expression and its functionality on cultured human MCs and conditioned basophils, that is, basophils with upregulated surface expression of MRGPRX2.
嗜碱性粒细胞和肥大细胞（MCs）是免疫系统中的重要效应细胞。长期以来，人们已知这些细胞可通过与高亲和力受体（FcεRI）结合的IgE抗体交联而被激活。然而，越来越多的证据表明，这些细胞也可通过多种不依赖IgE的机制被激活。占据G蛋白偶联受体MAS相关GPR家族成员X2（MRGPRX2）被描述为一种不依赖IgE的替代激活机制。在此，我们描述一种流式细胞术技术，用于分析培养的人MCs和条件嗜碱性粒细胞（即表面MRGPRX2表达上调的嗜碱性粒细胞）上MRGPRX2的表达及其功能。
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