肥大细胞活化试验 (MAT)。
Mast Cell Activation Test (MAT).
机构信息
Lydia Becker Institute of Immunology and Inflammation, Division of Musculoskeletal and Dermatological Sciences & Manchester Collaborative Centre for Inflammation Research (MCCIR), School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK.
出版信息
Methods Mol Biol. 2020;2163:227-238. doi: 10.1007/978-1-0716-0696-4_19.
The mast cell (MC) activation assay is a robust in vitro tool for exploring MC reactivity in allergy. Here we describe the use of the mast cell activation test (MAT) that makes use of human primary MCs generated from peripheral blood progenitors, sensitized overnight with patients' sera and activated with allergens. Flow cytometry is used to assess the changes in expression of the activation marker CD63, and the percentage of cell degranulation is defined as the percentage of CD63-positive MCs.
肥大细胞（MC）激活测定是一种强大的体外工具，用于研究过敏中的 MC 反应性。在这里，我们描述了肥大细胞激活试验（MAT）的使用，该试验利用从外周血祖细胞生成的人原代 MC，通过患者血清进行过夜致敏，并通过过敏原进行激活。流式细胞术用于评估激活标志物 CD63 的表达变化，脱颗粒细胞的百分比定义为 CD63 阳性 MC 的百分比。
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