Laboratory of Signal Transduction, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.
Department of Pathology and Immunology, Washington University in St. Louis, St. Louis, MO, USA.
Methods Mol Biol. 2020;2163:293-310. doi: 10.1007/978-1-0716-0696-4_24.
A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter, three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.
更好地理解导致肥大细胞迁移和趋化的分子机制是肥大细胞研究的长期目标，对于理解肥大细胞在健康和疾病中的功能至关重要。近年来，已经开发出各种用于体外和体内评估肥大细胞运动性和趋化性的技术。本章介绍了三种有助于实时定量测定肥大细胞趋化性和迁移的显微镜检测方法，重点介绍了单个细胞的跟踪和数据分析。
