Webster L M, Thomson A W
Department of Pathology, University of Aberdeen, Scotland, U.K.
J Immunol Methods. 1988 Feb 10;106(2):277-81. doi: 10.1016/0022-1759(88)90208-6.
Footpad swelling in response to subcutaneous antigen challenge is widely used to assess delayed-type hypersensitivity responses to xenogeneic erythrocytes in mice. We report here that quantitation of the deposition of 125I-fibrin, compared with that of 125I-human serum albumin, following intravenous isotope administration, provides a less subjective, convenient and antigen specific index of cell-mediated immunity. Moreover, the results obtained correlate well with in vitro assessment of antigen-specific T cell responses.
足垫对皮下抗原攻击的肿胀反应被广泛用于评估小鼠对异种红细胞的迟发型超敏反应。我们在此报告，静脉注射同位素后，与125I-人血清白蛋白相比，对125I-纤维蛋白沉积的定量提供了一种主观性较小、方便且具有抗原特异性的细胞介导免疫指标。此外，所获得的结果与抗原特异性T细胞反应的体外评估结果相关性良好。
