Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE International Joint Collaborative Research Laboratory for Animal Health & Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
Vet Microbiol. 2020 Aug;247:108753. doi: 10.1016/j.vetmic.2020.108753. Epub 2020 Jun 20.
Senecavirus A (SVA), previously called Seneca Valley virus, belongs to the family Picornaviridae, species Senecavirus A, in the Senecavirus genus, and can cause vesicular lesions in sows and acute death in piglets. In this study, recombinant VP1 and VP2 proteins were expressed in prokaryotic expression system and used to generate eight monoclonal antibodies (mAbs) against VP1 or VP2 protein. And all of the mAbs reacted specifically with SVA virus by both Western blot and indirect immunofluorescence assay (IFA). The resurts showed that all of the epitopes aganist these mAbs were B cell linear epitopes. To map the epitopes, both Western blot and indirect enzyme-linked immunosorbant assay (indirect ELISA) were performed. The epitope GELAAP recognized by mAb 1G9, was likely to be a significant B cell epitope due to the high antigenic index and the fully exposure on the surface of the VP1. Other mAbs were recognized by VP2 protein. MAbs 1E7 and 8E8 recognized the same epitope at DRVITQT, 1A5 recognized the epitope at WTKAVK, 1G2 recognized the epitope at GGAFTA, 9D2 and 6B11 recognized the same epitope at KSLQELN, and 7E4 recognized the epitope at YKEGAT. Alignment of amino acids revealed that four epitopes were completely conserved among all SVA strains, including GELAAP, WTKAVK, GGAFTA, and YKEGAT. Interestingly, there were some amino acid mutations in DRVITQT and KSLQELN, but no significant difference was detected on the reaction intensity between epitopes and the corresponding mAbs. This is the first report about the SVA epitopes, which will benefit to the study of viral pathogenic mechanism, vaccine design, as well as the establishment of detection methods.
猪传染性胃肠炎病毒（TGEV）属于冠状病毒科冠状病毒属，是引起猪传染性胃肠炎的病原体。该病毒基因组为单股正链 RNA，全长 27.5～30kb，是目前已知的 RNA 病毒中基因组最大的病毒之一。
