Department of Dermatology, Wuhan No.1 Hospital, Hospital of Traditional Chinese and Western Medicine Affiliated to Hubei University of Chinese Medicine, Wuhan Hospital of Traditional Chinese and Western Medicine Affiliated to Huazhong University of Science and Technology, Wuhan, 430022, China.
Institute of Geriatrics, Hubei University of Chinese Medicine, Wuhan, 430065, China.
J Tissue Viability. 2020 Nov;29(4):354-358. doi: 10.1016/j.jtv.2020.06.001. Epub 2020 Jul 28.
This aim of this study was to observe the effect of Yang Yan Qing E Wan (YYQEW) on senescent phenotypes and the expression of β-catenin and p16 in the hydrogen peroxide (HO)-induced premature senescence of normal human skin fibroblasts (NHSFs). Primary normal human skin fibroblasts were randomly divided into a normal group, a blank group, a model group, and a YYQEW group. The cells of the model group and the YYQEW group were exposed to 150 μmol/L HO for 2 h. The morphological changes of the cells were analyzed by microscopy and by kits used to estimate the activities of the senescence-associated β-galactosidase (SA-β-gal), reactive oxygen species (ROS), and superoxide dismutase (SOD). The outcomes revealed that dyeing rate proportion of SA-β-gal was 2.78% ± 0.22% in the normal group, 2.83% ± 0.29% in the blank group, 37.58% ± 2.56% in the model group, and 28.39% ± 0.93% in the YYQEW group. The number of SA-β-gal positive cells was thus significantly higher in the model group than in the normal or blank group. There were also fewer SA-β-gal positive cells in the YYQEW group compared with the model group. The expression of ROS and p16 in the model group increased significantly compared with that in the normal or blank groups, while the expression of ROS and p16 in the YYQEW group decreased significantly compared with that in the model group. The expression of SOD and β-catenin in the model group decreased significantly compared with that in the normal or blank group, and the expression of SOD and β-catenin in the YYQEW group increased significantly compared with that in the model group. Overall, it was found that YYQEW was able to delay the senescence of NHSFs induced by HO treatment by alleviating oxidative stress and regulating a number of senescence-related molecules, such as β-catenin and p16.
本研究旨在观察咽痒清丸（YYQEW）对过氧化氢（HO）诱导正常人皮肤成纤维细胞（NHSFs）衰老表型及β-catenin 和 p16 表达的影响。将原代正常人皮肤成纤维细胞随机分为正常组、空白组、模型组和 YYQEW 组。模型组和 YYQEW 组细胞用 150μmol/L HO 孵育 2 h。显微镜观察和试剂盒检测衰老相关β-半乳糖苷酶（SA-β-gal）、活性氧（ROS）和超氧化物歧化酶（SOD）活性，分析细胞形态变化。结果显示，正常组 SA-β-gal 染色率比例为 2.78%±0.22%，空白组为 2.83%±0.29%，模型组为 37.58%±2.56%，YYQEW 组为 28.39%±0.93%。模型组 SA-β-gal 阳性细胞数明显高于正常组和空白组，YYQEW 组 SA-β-gal 阳性细胞数明显少于模型组。与正常组或空白组相比，模型组 ROS 和 p16 表达明显增加，而 YYQEW 组 ROS 和 p16 表达明显降低。与正常组或空白组相比，模型组 SOD 和 β-catenin 表达明显降低，YYQEW 组 SOD 和 β-catenin 表达明显升高。综上所述，YYQEW 可通过减轻氧化应激和调节β-catenin 和 p16 等多种衰老相关分子，延缓 HO 诱导的 NHSFs 衰老。
