Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul, Republic of Korea.
Department of Food and Nutrition, College of Human Ecology, Seoul National University, Seoul, Republic of Korea; Research Institute of Human Ecology, College of Human Ecology, Seoul National University, Seoul, Republic of Korea.
J Nutr Biochem. 2020 Nov;85:108464. doi: 10.1016/j.jnutbio.2020.108464. Epub 2020 Jul 8.
Vitamin D has been reported to regulate the maturation and function of dendritic cells (DCs). Obesity was shown to be associated with the dysregulation of vitamin D metabolism and malfunction of DCs. We investigated the effects of in vitro 1,25(OH)D treatment (0, 1, or 10 nM) on phenotype and expression of genes related to function of bone marrow-derived DCs (BMDCs) from control and obese mice. C57BL/6 N mice were fed a control or high-fat (10% or 45% kcal fat: CON or HFD) diets for 15 weeks. Differentiation toward DCs was induced with GM-CSF (20 ng/ml) and maturation was induced by LPS (50 ng/ml); 10 nM 1,25(OH)D treatment inhibited BMDC differentiation (CD11c) and decreased the percentage of mature DCs (MHCIICD11c and CD86CD11c) in both CON and HFD groups. The Il10 expression in stimulated BMDCs from the CON group increased with the 10 nM 1,25(OH)D treatment, but not in those from the HFD group. The Il12b mRNA levels in stimulated BMDCs were lower in the HFD group than in the CON group. In conclusion, lower levels of Cd 40, Cd83 and Il12 mRNA in LPS-stimulated BMDCs from obese mice suggest malfunction of DCs as antigen presenting cells. 1,25(OH)D treatment inhibited the differentiation and maturation of BMDCs in both control and obese mice. Differential effects of 1,25(OH)D on the expression of Il10 between control and obese mice suggest that regulation of immune response by vitamin D could be influenced by obesity.
维生素 D 被报道可调节树突状细胞 (DC) 的成熟和功能。肥胖与维生素 D 代谢失调和 DC 功能障碍有关。我们研究了体外 1,25(OH)D 处理（0、1 或 10 nM）对对照和肥胖小鼠骨髓来源的 DCs (BMDCs) 表型和与功能相关基因表达的影响。C57BL/6 N 小鼠用对照或高脂肪（10%或 45%热量脂肪：CON 或 HFD）饮食喂养 15 周。用 GM-CSF（20 ng/ml）诱导向 DC 分化，用 LPS（50 ng/ml）诱导成熟；10 nM 1,25(OH)D 处理抑制 BMDC 分化（CD11c），并降低 CON 和 HFD 组中成熟 DC 的百分比（MHCIICD11c 和 CD86CD11c）。CON 组刺激的 BMDCs 中的 Il10 表达随 10 nM 1,25(OH)D 处理而增加，但 HFD 组则不然。刺激的 BMDCs 中的 Il12b mRNA 水平在 HFD 组中低于 CON 组。总之，肥胖小鼠 LPS 刺激的 BMDCs 中 Cd 40、Cd83 和 Il12 mRNA 水平较低提示 DC 作为抗原呈递细胞功能障碍。1,25(OH)D 处理抑制对照和肥胖小鼠 BMDC 的分化和成熟。1,25(OH)D 对对照和肥胖小鼠 Il10 表达的差异影响表明，维生素 D 对免疫反应的调节可能受肥胖影响。
