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大鼠表皮干细胞促进全层创面血管生成。

Rat epidermal stem cells promote the angiogenesis of full-thickness wounds.

机构信息

Department of Burn, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong Province, People's Republic of China.

Department of Plastic Surgery, Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong Province, People's Republic of China.

出版信息

Stem Cell Res Ther. 2020 Aug 8;11(1):344. doi: 10.1186/s13287-020-01844-y.

DOI:10.1186/s13287-020-01844-y
PMID:32771044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7414990/
Abstract

BACKGROUND

Full-thickness wounds severely affect patients' life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds.

METHODS

Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence.

RESULTS

The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing.

CONCLUSIONS

Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.

摘要

背景

全层创面严重影响患者的生活质量，成为临床医生面临的挑战。干细胞在创面治疗中具有广阔的前景。我们之前的研究证实，自体基底细胞悬液能促进创面愈合，且在基底细胞悬液中检测到表皮干细胞（ESCs）。本研究旨在探讨 ESCs 对全层创面的影响。

方法

分离和扩增大鼠 ESCs，然后用慢病毒转染使其稳定表达增强型绿色荧光蛋白。实验大鼠随机分为 2 组：在 ESCs 组中，将大鼠 ESCs 喷在大鼠全层创面；在对照组中，将磷酸盐缓冲液喷在创面。然后，评估创面愈合和新生血管形成情况。通过免疫荧光分析 ESCs 在创面的定植、分裂和分化情况。

结果

大鼠 ESCs 在创面中定植、分裂和增殖。此外，血管周围的大鼠 ESCs 分化为血管内皮细胞，并形成管腔样结构。与对照组相比，ESCs 组表现出更强的血管生成和更快的创面愈合。

结论

本研究证实大鼠 ESCs 治疗全层创面是安全有效的。此外，在一定条件下，ESCs 可分化为血管内皮细胞，促进血管生成和创面愈合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/2eefa265487b/13287_2020_1844_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/2b4e1f277522/13287_2020_1844_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/8ca38b644988/13287_2020_1844_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/c9c4dfe29120/13287_2020_1844_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/2eefa265487b/13287_2020_1844_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/2b4e1f277522/13287_2020_1844_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/8ca38b644988/13287_2020_1844_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/c9c4dfe29120/13287_2020_1844_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d19/7414990/2eefa265487b/13287_2020_1844_Fig4_HTML.jpg

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