You Yanjie, Bai Feihu, Li Haijun, Ma Yuhong, Yao Li, Hu Jinpeng, Tian Yonggang
Department of Gastroenterology, People's Hospital of Ningxia Hui Autonomous Region Yinchuan 750021, China.
Department of Radiation Oncology, The Second People's Hospital of Neijiang Neijiang 641003, Sichuan, China.
Am J Transl Res. 2020 Jul 15;12(7):3376-3388. eCollection 2020.
Despite significant advances in the early diagnosis and effective treatment of gastric cancer, it remains the fourth most common cancer and the second leading cause of cancer-related deaths worldwide. The zinc-fingers and homeoboxes (ZHX) family of transcriptional repressors has been shown to play a role in multiple types of cancer. However, the prognostic significance of ZHX expression in patients with gastric cancer remains unclear. This work studied the association between differential expression of ZHX mRNA and outcomes in patients with gastric cancer using data from the Oncomine, CCLE, Kaplan-Meier-plotter, and cBioPortal databases. Expression of ZHX3 protein was also measured by immunohistochemistry (IHC) in gastric cancer tissues. We found that increased expression of mRNA and decreased expression of and were correlated with better overall survival (OS) in patients with gastric cancer. Further subgroup analyses identified significant associations between expression and survival in select gastric cancer patients. IHC staining confirmed that the over-expression of ZHX3 was associated with worse OS, and multivariate analyses identified ZHX3 expression as an independent prognostic factor. These results suggest that the ZHX family members may serve as distinct biomarkers and prognostic factors for patients with gastric cancer.
尽管在胃癌的早期诊断和有效治疗方面取得了显著进展，但它仍是全球第四大常见癌症，也是癌症相关死亡的第二大主要原因。转录抑制因子锌指和同源框（ZHX）家族已被证明在多种类型的癌症中发挥作用。然而，ZHX表达在胃癌患者中的预后意义仍不清楚。这项工作使用来自Oncomine、CCLE、Kaplan-Meier-plotter和cBioPortal数据库的数据，研究了ZHX mRNA差异表达与胃癌患者预后之间的关联。还通过免疫组织化学（IHC）检测了胃癌组织中ZHX3蛋白的表达。我们发现，ZHX mRNA表达增加以及ZHX1和ZHX2表达降低与胃癌患者更好的总生存期（OS）相关。进一步的亚组分析确定了特定胃癌患者中ZHX1表达与生存之间的显著关联。免疫组织化学染色证实，ZHX3的过表达与较差的总生存期相关，多变量分析确定ZHX3表达为独立的预后因素。这些结果表明，ZHX家族成员可能作为胃癌患者独特的生物标志物和预后因素。
