外周血单个核细胞中 NF-B 激活与放疗后晚期皮肤和皮下组织纤维化的关系。

Association between NF-B Activation in Peripheral Blood Mononuclear Cells and Late Skin and Subcutaneous Fibrosis following Radiotherapy.

机构信息

Cancer Center, The First Hospital of Jilin University, 71 Xinmin Ave, Changchun, China.

Department of Radiation Oncology, The First Hospital of Jilin University, 71 Xinmin Ave, Changchun, China.

出版信息

Biomed Res Int. 2020 Jul 21;2020:2957818. doi: 10.1155/2020/2957818. eCollection 2020.

DOI:10.1155/2020/2957818

PMID:32775415

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7391119/

Abstract

BACKGROUND

This study aimed at evaluating the association between the speed of nuclear factor-kappa B (NF-B) activation in peripheral blood mononuclear cells (PBMCs) and late skin and subcutaneous fibrosis in patients with head and neck squamous cell carcinoma (HNSCC) after radiotherapy.

METHODS

The speed of NF-B activation was represented by the nuclear p65 expression ratio before and after irradiation. The optimal time point to measure the ratio was determined by Western blot in the PBMCs from healthy outpatients ranging from 0 to 12 hours after ex vivo irradiation. We recruited patients with HNSCC who had received ratiotherapy and who were under regular follow-up care. We assessed the association between the risk of developing ≥grade 2 late fibrosis and the nuclear p65 expression ratio in the PBMCs after ex vivo irradiation in these patients.

RESULTS

The maximum nuclear p65 ratio was observed at 1 hour after ex vivo irradiation in the PBMCs from the healthy outpatients. The speed of NF-B activation was then represented by the nuclear p65 ratio in the PBMCs before and 1 hour after ex vivo irradiation. A total of 200 patients with HNSCC were recruited, 32.50% ( = 65) of which presented with ≥grade 2 late fibrosis. There was a significant association between the speed of NF-B activation in the PBMCs and an increased risk of developing ≥grade 2 late fibrosis in these patients ( = 0.004). Subgroup analysis suggested that this finding was independent of the known clinical characteristics.

CONCLUSIONS

The speed of NF-B activation might be a potential predictor of late toxicity in cancer patients after radiotherapy. Prospective studies are needed for validation.

摘要

背景

本研究旨在评估头颈部鳞状细胞癌（HNSCC）患者放疗后外周血单个核细胞（PBMC）中核因子-κB（NF-κB）激活速度与皮肤和皮下组织迟发性纤维化之间的关系。

方法

NF-κB 激活速度用照射前后核 p65 表达比值表示。通过对健康门诊患者 PBMC 进行Western blot 分析，确定从照射后 0 至 12 小时之间测量比值的最佳时间点。我们招募了接受放疗并定期接受随访的 HNSCC 患者。我们评估了这些患者 PBMC 中体外照射后核 p65 表达比值与发生≥2 级迟发性纤维化风险之间的关系。

结果

健康门诊患者 PBMC 中，体外照射后 1 小时观察到最大核 p65 比值。然后，NF-κB 激活速度用 PBMC 中照射前和体外照射后 1 小时的核 p65 比值表示。共招募了 200 例 HNSCC 患者，其中 32.50%（=65）例出现≥2 级迟发性纤维化。PBMC 中 NF-κB 激活速度与这些患者发生≥2 级迟发性纤维化风险增加之间存在显著相关性（=0.004）。亚组分析表明，这一发现与已知的临床特征无关。