Kendal W S, Frost P
Department of Cell Biology, University of Texas M.D. Anderson Hospital and Tumor Institute, Houston 77030.
Cancer Res. 1988 Mar 1;48(5):1060-5.
Luria-Delbrück fluctuation analysis provides a method to estimate mutation rates in cell populations. Originally designed for bacterial populations, the method now is widely applied in somatic cell genetics and in cancer biology. However, there are fundamental genetic differences between bacteria and somatic cells, and this together with the inherent mathematical complexity of fluctuation analysis can lead to many pitfalls in its application. In addition there is considerable statistical error associated with the method. The use, misuse, and limitations of fluctuation analysis are reviewed here with the hope that such problems may be avoided.
鲁里亚-德尔布吕克波动分析提供了一种估计细胞群体中突变率的方法。该方法最初是为细菌群体设计的，现在广泛应用于体细胞遗传学和癌症生物学领域。然而，细菌和体细胞之间存在根本的遗传差异，再加上波动分析固有的数学复杂性，这可能导致其应用中出现许多陷阱。此外，该方法还存在相当大的统计误差。本文对波动分析的使用、误用及局限性进行了综述，希望能避免此类问题。
