University of Belgrade, Faculty of Chemistry, Studentski trg 16, 11000 Belgrade, Serbia.
University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Jan;239:108852. doi: 10.1016/j.cbpc.2020.108852. Epub 2020 Aug 8.
Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
近年来，由于管理的蜜蜂群体对经济和生态的重要性，蜜蜂群体的损失一直是研究的焦点。外部环境的不利条件对蜂群的健康有很大的影响。大多数损失主要发生在冬季，确切原因尚不完全清楚。只有少数研究涉及生物元素的含量、它们的功能以及它们对蜜蜂生理学的影响。本研究的目的是确定源自城市化和工业化程度不同的三个地区的蜜蜂血淋巴和整个机体中生物元素和非必需元素的季节性和空间变化。比较了来自三个不同环境的样本中的 16 种元素的浓度：常量元素（Ca、K、Mg、Na）、微量元素（Cu、Fe、Mn、Zn）和非必需元素（Al、Ba、Cd、Co、Cr、Ni、Pb、Sr）。在来自三个不同环境的样本中，比较了蜜蜂血淋巴和整个机体中的生物元素和非必需元素的含量：Golija（农村地区）、贝尔格莱德（城市地区）和 Zajača（工业地区）。环境、季节和蜜蜂活动程度对蜜蜂体内的生物元素和非必需元素含量有明显影响。血淋巴有助于区分空气污染与蜜蜂暴露的其他来源。我们的研究结果表明，蜜蜂可以成功地用作生物监测器，因为我们观察到观察到的地点之间存在统计学上的显著差异，但除非比较地点暴露于极其不同的污染压力之下，否则在同一季节收集所有蜜蜂至关重要。
