苦杏仁苷可降低 MDA-MB-231 和 MCF-7 乳腺癌细胞系的黏附和迁移。

Amygdalin Decreases Adhesion and Migration of MDA-MB-231 and MCF-7 Breast Cancer Cell Lines.

机构信息

Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Curr Mol Pharmacol. 2021 Oct 25;14(4):667-675. doi: 10.2174/1874467213666200810141251.

DOI:10.2174/1874467213666200810141251

PMID:32778045

Abstract

BACKGROUND

Cell adhesion, as dynamic interactions between cell-cell and cell-matrix, has an essential role in cancer cell migration. Integrins as cell membrane receptors are involved in cell adhesion and signal transduction. Aberrant expression of integrins is associated with the cancer cell adhesion.

OBJECTIVE

Targeting the process of cell adhesion and migration could be helpful to prevent cancer cell metastasis. Amygdalin is a cyanoglycoside compound with anti-cancer properties, while its effect on cancer cell adhesion is not completely clear.

METHODS

The cytotoxic effect of amygdalin on breast cancer cell lines (MCF-7 and MDA-MB- 231) and human skin fibroblast cell line as a normal cell, was evaluated through MTT assay. The cell adhesion assay and wound healing assay were performed to determine amygdalin effects on adhesion and migration of cancer cells. Further analysis was carried out to evaluate integrin α and β levels through real-time PCR.

RESULTS

We demonstrated that amygdalin diminished the cell viability of both cell lines in a time and dose-dependent manner, while amygdalin did not have any toxicity on the human skin fibroblast cell line in the same dosages. Following amygdalin treatment, the adhesion of both studied cell lines to fibronectin and collagen I decrease, and this reduction is significantly greater in the case of binding to fibronectin compared to binding to collagen. The MDA-MB-231 cell migration was decreased greater than MCF-7 cells. The levels of α and β integrin were differentially regulated by amygdalin in both cancer cell lines.

CONCLUSION

These results suggest that depending on cancer cell lines, amygdalin affects cancer cell adhesion and migration.

摘要

背景

细胞黏附作为细胞-细胞和细胞-基质之间的动态相互作用，在癌细胞迁移中起着重要作用。整合素作为细胞膜受体，参与细胞黏附和信号转导。整合素的异常表达与癌细胞黏附有关。

目的

靶向细胞黏附和迁移过程可能有助于预防癌细胞转移。苦杏仁苷是一种具有抗癌特性的氰糖苷化合物，但它对癌细胞黏附的影响尚不完全清楚。

方法

通过 MTT 法评估苦杏仁苷对乳腺癌细胞系（MCF-7 和 MDA-MB-231）和人皮肤成纤维细胞系（正常细胞）的细胞毒性作用。通过细胞黏附实验和划痕愈合实验来确定苦杏仁苷对癌细胞黏附和迁移的影响。进一步通过实时 PCR 分析来评估整合素α和β的水平。

结果

我们证明苦杏仁苷以时间和剂量依赖的方式降低了两种细胞系的细胞活力，而在相同剂量下，苦杏仁苷对人皮肤成纤维细胞系没有任何毒性。经苦杏仁苷处理后，两种研究细胞系与纤维连接蛋白和 I 型胶原的黏附均减少，而与纤维连接蛋白的结合比与胶原的结合减少更为显著。MDA-MB-231 细胞迁移减少大于 MCF-7 细胞。苦杏仁苷在两种癌细胞系中对α和β整合素水平的调节存在差异。