MYC Participates in Lipopolysaccharide-Induced Sepsis via Promoting Cell Proliferation and Inhibiting Apoptosis.

OBJECTIVE

This study aimed to explore the potential mechanism of proto-oncogene, BHLH Transcription Factor gene, on sepsis.

MATERIALS AND METHODS

In this experimental study, rat-derived H9C2 cardiomyocyte cells were cultured , followed by lipopolysaccharide (LPS) treatment with different concentration gradients. The cholecystokinin octapeptide (CCK-8) assay, enzyme-linked immunoassay (ELISA) assay, quantitative reverse transcription polymerase chain reaction (qRT-PCR), cell transfection, Western blot and flow cytometry were used to observe the cellular apoptosis and proliferation of cells in both treated LPS groups and normal control group.

RESULTS

The result of CCK-8 assay showed that silencing inhibited cellular proliferation of sepsis in absence or presence of LPS treatment. ELISA assay showed that the expressions of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were decreased in silenced group, but they were increased after LPS treatment. Moreover, Flow cytometry assay showed that silencing contributed to the apoptosis of sepsis cells. Furthermore, the expression of inflammatory factors showed that silencing elevated the expression of inflammation factors.

CONCLUSION

might take part in the process of LPS induced sepsis through suppressing apoptosis and inducing cell proliferation. Moreover, might reduce inflammation during the progression of LPS induced sepsis.