Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
School of Science and the Environment, Memorial University of NL (Grenfell Campus), Corner Brook, NL, A2H 5G4, Canada.
J Appl Microbiol. 2021 Mar;130(3):891-900. doi: 10.1111/jam.14821. Epub 2020 Sep 1.
To investigate the transcription of selected antioxidants and relevant genes under varying temperature conditions, and to identify the optimum temperature for antioxidants production by Arthrospira platensis.
The dry weight (DW), pigment production, antioxidants production and gene transcription were examined in A. platensis growing under three temperatures of 23, 30 and 37°C. The cyanobacterial DW was highest in the high temperatures (30 and 37°C), while the pigments, such as Chl a, carotenoids, C-phycocyanin and total phycobiliprotein contents, showed their maximum value at 30°C. The total soluble protein and carbohydrate contents were highest at 30°C. Lipid peroxidation, as a marker for thermal stress, was high at 23°C, while higher temperatures remarkably reduced lipid peroxidation levels. Antioxidants activity was increased by 1·5-fold at 30°C and temperature fluctuations induced the antioxidant enzyme activities. The transcriptional abundance of heat shock protein (HSP90), glutamate synthase (GOGAT), delta-9 desaturase (desC), iron-superoxide dismutase (FeSOD) and the large subunit of Rubisco (rbcL) genes was measured under the same temperatures.
The optimal temperature for growth, biochemical constituents and antioxidants of A. platensis is 30°C while some antioxidant enzyme activity increased at lower and higher temperatures.
The study showed the significance of temperature for growth, enzymatic and non-enzymatic antioxidants and gene expression in A. platensis. This contributes to the knowledge of culturing A. platensis to harvest specific antioxidants or as an antioxidant-rich food source.
研究在不同温度条件下选择的抗氧化剂和相关基因的转录情况，并确定钝顶螺旋藻生产抗氧化剂的最佳温度。
在 23、30 和 37°C 三种温度下培养钝顶螺旋藻，检测其干重（DW）、色素产生、抗氧化剂产生和基因转录情况。在高温（30 和 37°C）下，蓝藻 DW 最高，而色素（如 Chl a、类胡萝卜素、C-藻蓝蛋白和总藻胆蛋白）在 30°C 时达到最大值。总可溶性蛋白和碳水化合物含量在 30°C 时最高。作为热应激标志物的脂质过氧化在 23°C 时最高，而较高的温度则显著降低了脂质过氧化水平。抗氧化剂活性在 30°C 时增加了 1.5 倍，温度波动诱导了抗氧化酶的活性。在相同温度下测量了热休克蛋白（HSP90）、谷氨酸合酶（GOGAT）、δ-9 去饱和酶（desC）、铁超氧化物歧化酶（FeSOD）和 Rubisco 大亚基（rbcL）基因的转录丰度。
钝顶螺旋藻生长、生化成分和抗氧化剂的最佳温度为 30°C，而某些抗氧化酶活性在较低和较高温度下增加。
该研究表明温度对钝顶螺旋藻生长、酶和非酶抗氧化剂以及基因表达的重要性。这有助于了解培养钝顶螺旋藻以收获特定抗氧化剂或作为富含抗氧化剂的食物来源的知识。
