Andaman Nicobar Centre for Ocean Science and Technology, NIOT R&D Complex, Dollygunj, Port Blair 744103, A&N Islands, India.
Bioresour Technol. 2010 Dec;101(23):9221-7. doi: 10.1016/j.biortech.2010.06.120. Epub 2010 Jul 23.
The prospects of utilizing pretreated seawater for the culture of Arthrospira (Spirulina) platensis was evaluated under laboratory conditions with three seawater media and a control: (1) Zarrouk media (freshwater-control) (2) seawater media SW 1 (3) seawater media SW2 and (4) seawater media SW 3. The relative performance of these media were investigated with respect to their biomass production, pigment production (phycocyanin, lutein and betacarotene), and biochemical composition. A. platensis grown in media SW 2 had a biomass production (2.99+/-0.145 g L(-1)) comparable to that of control media (3.114+/-0.085 g L(-1)); highest specific growth rate (0.255 d(-1)) and lowest doubling time (2.720 days). Phycocyanin content of the cells grown in seawater media SW 3(81.85%) was closer to that of control. Similarly the purity ratio of phycocyanin produced from cells grown in seawater media SW 3 and control were closer to 4, while the phycocyanin obtained from cells grown in other two media exhibited lower purity ratios due to accumulation of lower molecular weight carbohydrates. The phycocyanin/Chl-a ratio and the betacarotene/Chl-a ratio of the cells grown in seawater media were higher than control. The lutein content of A. platensis cells grown in seawater media SW 2 was higher than that of control. The cells grown in seawater media had a slightly modified biochemical composition than the control with a higher carbohydrate and lower protein content. All the three seawater based media with fewer chemicals than the control (Zarrouk media) supported the growth of A. platensis as good as the control.
在实验室条件下，利用预处理海水评价了三种海水培养基和一种淡水对照培养基（1. Zarrouk 培养基（淡水对照）；2. SW1 海水培养基；3. SW2 海水培养基；4. SW3 海水培养基）对螺旋藻（蓝藻）的培养前景。研究了这些培养基相对于生物量生产、色素生产（藻蓝蛋白、叶黄素和β-胡萝卜素）和生化成分的相对性能。在培养基 SW2 中生长的 A. platensis 的生物量生产（2.99+/-0.145 g L(-1)）与对照培养基（3.114+/-0.085 g L(-1)）相当；最高比生长速率（0.255 d(-1)）和最短倍增时间（2.720 天）。在海水培养基 SW3 中生长的细胞的藻蓝蛋白含量（81.85%）更接近对照。同样，从海水培养基 SW3 和对照中生长的细胞产生的藻蓝蛋白的纯度比更接近 4，而从其他两种培养基中生长的细胞产生的藻蓝蛋白由于积累了较低分子量的碳水化合物，其纯度比较低。在海水培养基中生长的细胞的藻蓝蛋白/Chl-a 比和β-胡萝卜素/Chl-a 比高于对照。在海水培养基 SW2 中生长的 A. platensis 细胞的叶黄素含量高于对照。与对照相比，在海水培养基中生长的细胞的生化成分略有变化，碳水化合物含量较高，蛋白质含量较低。所有三种海水培养基（比对照培养基 Zarrouk 培养基）所含的化学物质都少于对照培养基，都能像对照培养基一样支持 A. platensis 的生长。
