Role of / and During Irradiation in a PMA-Induced Inflammatory Model.

Sphingosine kinase 1 () and heat shock protein 27 () are important for antioxidant and anti-inflammatory effects after red light irradiation in an inflammatory model. The purpose of the present study was to evaluate whether and work independently or are dependent on some other regulator after 625 nm light-emitting diode irradiation in the human keratinocyte (HaCaT) cell line. Differentially expressed genes (DEGs) were identified between groups with or without 625 nm photobiomodulation (PBM) in the inflammatory model. Potential transcription factors (TFs) of key DEGs were predicted using the iRegulon plugin. The mechanism was investigated by analyzing mRNA and protein expression levels, prostaglandin E2 levels, and intracellular reactive oxygen species (ROS) in phorbol 12-myristate 13-acetate (PMA)-induced HaCaT cells after 625 nm PBM. A total of 6 TFs (e.g., ) and 51 key DEGs (e.g., ) were identified after 625 nm PBM in PMA-stimulated HaCaT cells. worked as a regulator of ; however, it did not affect . knockdown drastically decreased the expression level and increased the intracellular ROS, as well as the expression levels of inflammation-related proteins in PMA-induced HaCaT cells. In addition, the inhibition of decreased the anti-inflammatory effect of 625 nm PBM. worked as a TF of and exhibited anti-inflammatory and antioxidative effects through in PMA-induced HaCaT cells after 625 nm PBM. is essential for the 625 nm PBM-induced anti-inflammatory function. Therefore, / and could be used as potential biomarkers for anti-inflammatory therapy with 625 nm PBM.