Wang Lin-Ang, Yang Bo, Tang Tang, Yang Yuxin, Zhang Dianzheng, Xiao Hualiang, Xu Jing, Wang Luofu, Lin Li, Jiang Jun
Department of Urology, Daping Hospital/Army Medical Center of the PLA, Army Medical University, Chongqing 400042, P.R. China.
Cancer Center, Daping Hospital/Army Medical Center of the PLA, Army Medical University, Chongqing 400042, P.R. China.
Oncol Lett. 2020 Sep;20(3):2881-2887. doi: 10.3892/ol.2020.11814. Epub 2020 Jul 6.
The present study sought to estimate the applicability of apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1), vascular endothelial growth factor A (VEGFA) expression and CD163 tumor-associated macrophage (TAM) ratio as prognostic factors in bladder cancer (BCa). A total of 127 patients with bladder urothelial cancer who underwent radical cystectomy at Daping Hospital were recruited between January 2013 and January 2017, including 45 cases of non-muscle invasive BCa (NMIBC) and 82 of MIBC. Immunohistochemical detection of APE1, VEGFA and CD163, as well as multiple immunofluorescence staining for APE1, VEGFA, CD163 and CD34, were performed on tissue samples. For APE1 and VEGFA, the staining was graded based on intensity (0-3), while CD163 was graded (0-3) based on the percentage of positively stained cells. The prognostic value of APE1, VEGF and CD163 was assessed using Kaplan-Meier and Cox regression analysis. The results suggested that in BCa, high APE1 expression was associated with high VEGFA expression and more infiltration of CD163 TAM. Furthermore, high expression of APE1 was associated with lymphovascular invasion of BCa, as well as reduced survival time. This indicates that APE1 may be associated with CD163 TAM infiltration in BCa, with VEGFA as a possible influencing factor.
本研究旨在评估脱嘌呤/脱嘧啶核酸内切酶1（APE1）、血管内皮生长因子A（VEGFA）表达以及CD163肿瘤相关巨噬细胞（TAM）比例作为膀胱癌（BCa）预后因素的适用性。2013年1月至2017年1月期间，共招募了127例在大坪医院接受根治性膀胱切除术的膀胱尿路上皮癌患者，其中非肌层浸润性BCa（NMIBC）45例，肌层浸润性BCa（MIBC）82例。对组织样本进行APE1、VEGFA和CD163的免疫组织化学检测，以及APE1、VEGFA、CD163和CD34的多重免疫荧光染色。对于APE1和VEGFA，根据染色强度分级（0 - 3级），而CD163根据阳性染色细胞百分比分级（0 - 3级）。使用Kaplan - Meier和Cox回归分析评估APE1、VEGF和CD163的预后价值。结果表明，在BCa中，APE1高表达与VEGFA高表达以及CD163 TAM浸润增加相关。此外，APE1高表达与BCa的淋巴管浸润以及生存时间缩短相关。这表明APE1可能与BCa中CD163 TAM浸润相关，VEGFA可能是一个影响因素。
