6760Washington State University, Pullman, WA, USA.
Vet Pathol. 2020 Nov;57(6):821-824. doi: 10.1177/0300985820948819. Epub 2020 Aug 12.
A 6-year-old, spayed female Labrador/Weimaraner cross-breed dog that had previously lived in Arizona presented in Montana for an annual examination with an incidentally enlarged popliteal lymph node, which was subsequently biopsied. Histologically, the lymph node was expanded by eosinophil-rich granulomas with both extracellular and intrahistiocytic green algae. These algae had intracytoplasmic, birefringent, and refractile granules; readily formed 2 to 3 mm green colonies on Columbia blood agar medium; and ultrastructurally had a multilayered cell wall and intracytoplasmic chloroplasts. Amplified product from the internal transcribed spacer and D1/D2 regions of the 28S ribosomal RNA gene had high sequence identity to sp. Despite similar infection in the retropharyngeal lymph node 1 year later, the animal remained otherwise healthy with no clinical signs. To the authors' knowledge, this is the first case of species infection in a dog and is a differential diagnosis for .
一只 6 岁、已绝育的雌性拉布拉多/威玛猎犬杂交犬，曾居住在亚利桑那州，因偶然发现的腘窝淋巴结肿大而在蒙大拿州进行年度检查，随后对该淋巴结进行了活检。组织学上，淋巴结被富含嗜酸性粒细胞的肉芽肿所扩张，既有细胞外的也有组织内的绿藻。这些藻类具有胞内双折射和折射性颗粒；在哥伦比亚血琼脂培养基上容易形成 2 到 3 毫米的绿色菌落；超微结构具有多层细胞壁和胞内叶绿体。来自内部转录间隔区和 28S 核糖体 RNA 基因的 D1/D2 区的扩增产物与 sp. 具有高度的序列同一性。尽管 1 年后在咽后淋巴结中出现了类似的感染，但该动物仍然保持健康，没有任何临床症状。据作者所知，这是首例犬感染 种的病例，也是 的鉴别诊断。
