Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
Molecules. 2020 Aug 8;25(16):3605. doi: 10.3390/molecules25163605.
is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of from different habitats has shown significant differences. However, the molecular differences in from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of and the protein mechanism governing differences in quality.
续断是一种具有有益健康特性的中草药。迄今为止，不同产地的续断质量已经表现出显著差异。然而，不同产地续断的分子差异尚不清楚。本研究旨在研究不同产地续断的蛋白质水平差异。采用相对和绝对定量同位素标记（iTRAQ）和 2DLC/MS/MS 检测不同产地续断的统计学显著变化。通过蛋白质组学分析，共鉴定出 2149 种蛋白质，其中筛选出 42 种重要差异表达蛋白。通过对差异蛋白进行深入分析，发现湖北省续断的蛋白质代谢能量和碳水化合物代谢能力较强，但抗氧化能力较弱。我们发现，三个蛋白质，UTP-葡萄糖-1-磷酸尿苷酰转移酶、丙烯氧化物环化酶和异戊二烯二磷酸异构酶 2，可能是参与续断皂苷 VI 合成的关键蛋白。还发现了 8 种蛋白质在不同产地的续断中对环境胁迫做出响应。实时定量 PCR 分析证实了蛋白质组学分析的准确性和真实性。本研究结果可为探索不同产地续断次生代谢物差异的原因以及控制质量差异的蛋白质机制提供基础信息。
