Monnier J, Tognetti L, Miyamoto M, Suppa M, Cinotti E, Fontaine M, Perez J, Orte Cano C, Yélamos O, Puig S, Dubois A, Rubegni P, Del Marmol V, Malvehy J, Perrot J L
Department of Dermatology, Aix-Marseille University, Marseille, France.
Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, University of Siena, Siena, Italy.
J Eur Acad Dermatol Venereol. 2020 Dec;34(12):2914-2921. doi: 10.1111/jdv.16857. Epub 2020 Aug 27.
Line-field confocal optical coherence tomography (LC-OCT) is a non-invasive optical technique recently developed for skin examination in vivo. It provides real-time, high-resolution vertical images with an isotropic resolution of ~1 µm and a penetration depth of ~500 µm.
Study goals were to qualitatively/quantitatively characterize healthy skin at different body sites using LC-OCT.
The skin of young healthy volunteers was imaged with a handheld LC-OCT imaging device. Seven body sites (back of the hand, forehead, cheek, nose, chest, forearm and back) were investigated. An independent qualitative [cutaneous structures' description; visibility of keratinocytes' nuclei and dermal-epidermal junction (DEJ)] and quantitative [stratum corneum (SC)/epidermal thicknesses; height of dermal papillae] assessment of the LC-OCT images was performed.
A total of 88 LC-OCT images were collected from 29 participants (20 females; nine males; mean age 25.9 years). Keratinocytes' nuclei and DEJ were visible in the totality of images. The different layers of the epidermis and the remaining cutaneous structures/findings were visualized. Body sites-related variability was detected for SC/epidermal thicknesses and height of dermal papillae. Inter-observer agreement was excellent (SC thickness), good-to-excellent (epidermal thickness) and moderate-to-good (papillae).
Line-field confocal-OCT provides non-invasive, real-time imaging of the skin in vivo with deep penetration and high resolution, enabling the visualization of single cells. The histology-like vertical view provides an easy way to recognize/measure different cutaneous structures/findings. LC-OCT appears as a promising technique for the examination of physiological/pathological skin.
线场共聚焦光学相干断层扫描（LC-OCT）是一种最近开发的用于体内皮肤检查的非侵入性光学技术。它能提供实时、高分辨率的垂直图像，各向同性分辨率约为1微米，穿透深度约为500微米。
研究目标是使用LC-OCT对不同身体部位的健康皮肤进行定性/定量表征。
使用手持式LC-OCT成像设备对年轻健康志愿者的皮肤进行成像。研究了七个身体部位（手背、前额、脸颊、鼻子、胸部、前臂和背部）。对LC-OCT图像进行了独立的定性[皮肤结构描述；角质形成细胞核和真皮-表皮交界处（DEJ）的可见性]和定量[角质层（SC）/表皮厚度；真皮乳头高度]评估。
共从29名参与者（20名女性；9名男性；平均年龄25.9岁）收集了88张LC-OCT图像。在所有图像中均可见角质形成细胞核和DEJ。表皮的不同层以及其他皮肤结构/发现均清晰可见。检测到SC/表皮厚度和真皮乳头高度存在身体部位相关的变异性。观察者间一致性良好（SC厚度）、良好至优秀（表皮厚度）和中等至良好（乳头）。
线场共聚焦-OCT提供了一种非侵入性、实时的体内皮肤成像方法，具有深度穿透和高分辨率，能够可视化单个细胞。类似组织学的垂直视图为识别/测量不同的皮肤结构/发现提供了一种简便方法。LC-OCT似乎是一种用于检查生理性/病理性皮肤的有前景的技术。
