da Silva Severino Jefferson Ribeiro, Silva Caroline Targino Alves da, Guarines Klarissa Miranda, Mendes Renata Pessôa Germano, Pardee Keith, Kohl Alain, Pena Lindomar
Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), 50670-420, Recife, Pernambuco, Brazil.
Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.
ACS Infect Dis. 2020 Sep 11;6(9):2319-2336. doi: 10.1021/acsinfecdis.0c00274. Epub 2020 Aug 20.
In December 2019, a novel beta (β) coronavirus eventually named SARS-CoV-2 emerged in Wuhan, Hubei province, China, causing an outbreak of severe and even fatal pneumonia in humans. The virus has spread very rapidly to many countries across the world, resulting in the World Health Organization (WHO) to declare a pandemic on March 11, 2020. Clinically, the diagnosis of this unprecedented illness, called coronavirus disease-2019 (COVID-19), becomes difficult because it shares many symptoms with other respiratory pathogens, including influenza and parainfluenza viruses. Therefore, laboratory diagnosis is crucial for the clinical management of patients and the implementation of disease control strategies to contain SARS-CoV-2 at clinical and population level. Here, we summarize the main clinical and imaging findings of COVID-19 patients and discuss the advances, features, advantages, and limitations of different laboratory methods used for SARS-CoV-2 diagnosis.
2019年12月，一种最终被命名为严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的新型β冠状病毒在中国湖北省武汉市出现，导致人类爆发严重甚至致命的肺炎。该病毒已迅速传播到世界许多国家，致使世界卫生组织（WHO）于2020年3月11日宣布其为大流行病。临床上，这种前所未有的疾病被称为冠状病毒病2019（COVID-19），其诊断变得困难，因为它与包括流感病毒和副流感病毒在内的其他呼吸道病原体有许多共同症状。因此，实验室诊断对于患者的临床管理以及在临床和人群层面实施控制SARS-CoV-2的疾病控制策略至关重要。在此，我们总结了COVID-19患者的主要临床和影像学表现，并讨论了用于SARS-CoV-2诊断的不同实验室方法的进展、特点、优点和局限性。
