来自受刺激单核白细胞的条件培养基增强了人类中性粒细胞损伤人类关节软骨的能力。
Conditioned medium from stimulated mononuclear leucocytes potentiates the ability of human neutrophils to damage human articular cartilage.
作者信息
Bates E J, Kowanko I C, Ferrante A
机构信息
University Department of Paediatrics, Adelaide Children's Hospital, South Australia.
出版信息
Ann Rheum Dis. 1988 Jan;47(1):1-9. doi: 10.1136/ard.47.1.1.
Abstract
Human neutrophils were able to degrade proteoglycan and inhibit its synthesis when incubated with human articular cartilage coated with heat aggregated immunoglobulin G. These effects were potentiated when culture medium conditioned by mononuclear leucocytes stimulated with killed Staphylococcus aureus was also present during the incubations. Neutrophils preincubated with this conditioned medium and washed before incubation with cartilage also showed an increased ability to degrade proteoglycan and inhibit its synthesis. The percentage of neutrophils binding to cartilage was significantly increased in the presence of this conditioned medium.
摘要
当人中性粒细胞与包被有热聚集免疫球蛋白G的人关节软骨一起孵育时，它们能够降解蛋白聚糖并抑制其合成。当在孵育过程中同时存在由经热杀死的金黄色葡萄球菌刺激的单核白细胞调节的培养基时，这些作用会增强。用这种调节培养基预孵育并在与软骨孵育前洗涤过的中性粒细胞，其降解蛋白聚糖和抑制其合成的能力也有所增强。在这种调节培养基存在的情况下，中性粒细胞与软骨结合的百分比显著增加。
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本文引用的文献
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