Key Laboratory of Functional Molecular Engineering of Guangdong Province, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China.
J Am Chem Soc. 2020 Aug 19;142(33):14023-14027. doi: 10.1021/jacs.0c00443. Epub 2020 Aug 11.
Realizing robust DNA functionalization with strict valence control in the sub-2-nm thiolate-protected luminescent gold nanoparticles (AuNPs) is highly demanded but remains unsolved due to their unique Au(0) core and Au(I)-S shell structures. Herein, we report a facile strategy using phosphorothioates (ps)-modified DNA (psDNA) as a template for growth of near-infrared (NIR)-emitting AuNPs with precisely controlled DNA valence. In addition, the particle size could be finely tuned in ultrasmall ranges from 1.3 to 2.6 nm with regulation of the ps length of psDNA. The ultrasmall NIR-emitting AuNPs bearing strict DNA valence are also demonstrated to be as powerful building block for well-organized one-dimensional assembly and optical probe for targeted cellular imaging. Such a facile strategy in decoration of luminescent AuNPs with strict DNA valence provides a new pathway for development of surface-functionalizable ultrasmall metal nanoplatforms toward various downstream applications.
实现亚 2nm 巯基保护的发光金纳米粒子（AuNPs）中具有严格价态控制的稳健 DNA 功能化，由于其独特的 Au(0)核和 Au(I)-S 壳结构，这一需求非常高，但仍然未得到解决。在此，我们报告了一种使用磷硫代酸酯（ps）修饰的 DNA（psDNA）作为模板，用于生长近红外（NIR）发射的 AuNPs 的简便策略，可精确控制 DNA 的价态。此外，通过调节 psDNA 的 ps 长度，可以在超小范围内精细调节粒径，范围从 1.3nm 至 2.6nm。具有严格 DNA 价态的超小 NIR 发光 AuNPs 也被证明是用于一维有序组装的有力构建块，以及用于靶向细胞成像的光学探针。这种在发光 AuNPs 上进行严格 DNA 价态修饰的简便策略，为开发具有各种下游应用的表面功能化的超小金属纳米平台提供了新途径。
