Biochemistry Department, Faculty of Science, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia. E-mail.
Saudi Med J. 2020 Aug;41(8):834-840. doi: 10.15537/smj.2020.8.25187.
To measure the blood expression levels of related drug-resistant ATP-binding cassette (ABC) transporters in colorectal cancer (CRC) patients and to assess these examined transporters for whether they present signi cant expression in connection with the tumor appearance of CRC.
In this case-control study, the messenger ribonucleic acids were isolated from the blood of 62 CRC patients who were recruited from King Abdulaziz University Hospital Oncology Clinic and 46 controls from King Fahad General Hospital Blood Bank (Jeddah, Saudi Arabia) from September 2016 to March 2017. The Biomedical Ethics Unit at King Abdulaziz University, Jeddah, Saudi Arabia approved this study. The expressions of ABC transporters were measured using quantitative polymerase chain reaction. GraphPad Prism 5 and REST 2009 Software were used to correlate the expressions with clinicopathological independent stages and body mass index. A p-value of less than 0.05 was considered significant.
The results showed that the 3 ABC transporters, particularly ABCC1 (p less than 0.0001), were highly expressed in the blood of CRC patients compared with controls. However, none of the 3 transporters was related to the progression of CRC, age, gender, or body mass index.
The expressions of ABC transporters were found to be significantly higher in CRC patients, and they may act as diagnostic markers and should potentially be tested for their contribution to drug sensitivity in CRC patients.
检测结直肠癌（CRC）患者血液中相关耐药 ATP 结合盒（ABC）转运蛋白的表达水平，并评估这些检测到的转运蛋白在 CRC 肿瘤表现中是否存在显著表达。
在这项病例对照研究中，我们从 2016 年 9 月至 2017 年 3 月在沙特阿拉伯吉达阿卜杜勒阿齐兹国王大学医院肿瘤学诊所招募的 62 例 CRC 患者和 46 例来自沙特阿拉伯法赫德国王综合医院血库的对照者的血液中分离出信使核糖核酸。沙特阿拉伯吉达阿卜杜勒阿齐兹国王大学的生物医学伦理委员会批准了这项研究。使用定量聚合酶链反应测量 ABC 转运蛋白的表达。GraphPad Prism 5 和 REST 2009 软件用于将表达与临床病理独立分期和体重指数相关联。p 值小于 0.05 被认为具有统计学意义。
结果显示，与对照组相比，CRC 患者血液中的 3 种 ABC 转运蛋白，特别是 ABCC1（p 小于 0.0001），表达水平显著升高。然而，这 3 种转运蛋白均与 CRC 的进展、年龄、性别或体重指数无关。
我们发现 ABC 转运蛋白在 CRC 患者中的表达显著升高，它们可能作为诊断标志物，并可能需要对其在 CRC 患者中的药物敏感性贡献进行测试。
