Medina-Flores Fernanda, Hurtado-Alvarado Gabriela, Gómez-González Beatriz
Area of Neurosciences, Department Biology of Reproduction, CBS, Mexico City, Mexico.
Posgrado en Biología Experimental, Universidad Autónoma Metropolitana, Unidad Iztapalapa, Mexico City, Mexico.
Methods Mol Biol. 2021;2367:37-46. doi: 10.1007/7651_2020_313.
The study of the regionalized function of the blood-brain barrier at the level of brain endothelial cells and pericytes is essential to understand the biological properties and molecular mechanisms regulating this biological barrier. The isolation of blood vessels from specific brain regions will allow to understand regional differences in susceptibility to pathological phenomena such as ischemia, traumatic brain injury, and neurodegenerative diseases, such as Alzheimer disease. Here, we propose an efficient and fast method to isolate brain endothelial cells and pericytes from a specific cerebral region. The isolated brain endothelial cells and pericytes are viable to perform conventional molecular and histological techniques such as Western blots, immunocytofluorescence, and scanning electron microscopy.
在脑内皮细胞和周细胞水平上研究血脑屏障的区域化功能，对于理解调节这一生物屏障的生物学特性和分子机制至关重要。从特定脑区分离血管将有助于了解对缺血、创伤性脑损伤和神经退行性疾病（如阿尔茨海默病）等病理现象易感性的区域差异。在此，我们提出一种高效快速的方法，用于从特定脑区分离脑内皮细胞和周细胞。分离出的脑内皮细胞和周细胞可用于进行常规分子和组织学技术，如蛋白质免疫印迹、免疫细胞荧光和扫描电子显微镜检查。
